Examples of 'salt gradient' in a sentence
Meaning of "salt gradient"
salt gradient ~ a measurement of the rate of change in salt concentration over a given distance, often used in scientific contexts such as oceanography or chemistry
How to use "salt gradient" in a sentence
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salt gradient
The salt gradient is indicated by the thin line.
A simple device can be used to create a salt gradient.
A descending salt gradient may be used to elute the sample.
Plasmid was eluted with a salt gradient.
A stepped salt gradient was used.
Elute the bound enzyme with linear salt gradient.
Elution with a salt gradient makes it possible to recover the proteins.
The proteins were eluted form the column by an isocrational salt gradient.
The conditions of salt gradient are specific for the column used.
The DsbA is eluted from the column with a salt gradient.
Preferably, no salt gradient is used to elute the antibody.
The remaining bound protein may be eluted with a salt gradient.
The process of the invention uses a decreasing salt gradient to elute pneumolysin from the column.
The protein was eluted from the column using a stepwise salt gradient.
Protein was eluted with an increasing salt gradient and fractions collected.
See also
Each antigen was eluted off the column with an increasing salt gradient.
In further embodiments, the salt gradient is a linear gradient.
The polypeptide or immunoconjugate of the invention is eluted with a linear or step salt gradient.
In further embodiments, the salt gradient is a step gradient.
The column is washed with loading buffer and then eluted with a salt gradient.
Usually, a decreasing salt gradient is used to elute samples from the column.
Factor XIII is eluted from the column using a descending salt gradient.
After applying salt gradient described in example 6 all proteins are selectively eluted.
CTGF was eluted from the column with an increasing NaCl salt gradient.
A continuous linear salt gradient was applied to the column over 15 column volumes.
Elution of a DEAE column by introduction of a linear salt gradient gave three major peaks.
A three segment salt gradient is illustrated in FIG . 3 and described in more detail below.
Elution of the bound FIX or FIX fusión polypeptides was achieved by running a salt gradient.
An elution salt gradient is used of about 0 to 1M.
In some embodiments, the DsbA is eluted from the cation chromatography material using a salt gradient.
The salt gradient was followed by strip steps with water followed by 0.5N NaOH.
The isoforms of transferrin were separated in a two-buffer system with a multi-step salt gradient elution.
When the salt gradient reaches a concentration of about 0.4 M, the eluate contains essentially only Factor IX.
In addition, vpr protein was collected off a DEAE sepharose column using a salt gradient.
Proteins were eluted with a linear salt gradient in Buffer A from 50mM to 1M NaCl.
The dialyzed protein was then loaded onto a Q-sepharose column and eluted using a salt gradient.
In a preferred embodiment, a continuous salt gradient is one which ranges from 0 to 1000 mM NaCl.
The process of claim 1, wherein eluting comprises a decreasing salt gradient.
A used salt gradient may extend up to 2-3 M salt.
The bound activity was eluted with a linear salt gradient up to 1 M NaCI.
The method of Claim 6 wherein the gradient of step ( c ) is a salt gradient.
Bound proteins were subjected to a linear salt gradient with buffer B = A+100 mM NaCl.
PSP / reg is preferably purified using SP-Sepharose-cellulose by a pH and salt gradient.
Recombinant human GDNF was eluted from the column using a salt gradient from 0.3 M to 0.6 M sodium chloride.
PSP / reg is preferable purified using SP-Sepharose-cellulose by a pH and salt gradient.
The peak of trypsin, BoNT/E1 and the salt gradient are marked.
Eluting the PBP 2as from the dye-ligand resin with a linear salt gradient.
Peak activity eluted with a linear salt gradient at 0.2M NaCl.
Elution can be achieved, for example, using a rising salt gradient.
Which is known as “ osmotic pressure ”, it upturns the more imbalanced the salt gradient gets.
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