Examples of 'second amplification' in a sentence
Meaning of "second amplification"
second amplification: Refers to the process of further increasing the amplification or volume of something for a second time
How to use "second amplification" in a sentence
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second amplification
The second amplification ratio is practically constant.
The reaction mixture ordinarily includes first and second amplification primers.
Second amplification compartment in the third level.
This method has the disadvantage that it needs additional reagents for the second amplification.
A second amplification primer then binds to the cDNA.
Means for modulating the second amplification optical signal ;.
The second amplification step is advantageously effected in gain saturation mode.
The pairs of primers used for the second amplification are,.
A second amplification stage is catalysis of the indicator reaction by the second enzyme.
C-band signals are not amplified in the second amplification stage.
A second amplification primer that hybridizes to the anchor sequence under the annealing conditions.
Its second pole is connected to the drain of the second amplification transistor 20.
The second amplification can occur when a sample nucleic hybridizes to the newly generated strand.
Means for separating said first and a second amplification optical signal ;.
The second amplification stage comprises third and fourth MOS transistors of a second conductivity type.
See also
In this method, two NSIs are included in a first and second amplification primer.
Preferably, the second amplification product is quantified using a detection probe.
Each newly-synthesized amplicon can anneal with the second amplification primer.
In another aspect of the invention, a second amplification reaction is carried out as a confirmatory test.
The second amplification primer consists essentially of a target binding sequence of SEQ ID NO, 2.
However, smaller primers can be used in the second amplification and provide the requisite specificity.
The second amplification amplifies the rescued amplicons using fresh buffer, nucleotides, and common primer ( s ).
In addition, the well confined, compact second amplification volume leads to a highly sensitive optical setup.
A second amplification of the electric pulses ( " I " ) the frequency of which corresponds to the predetermined value ;.
An interface circuit as claimed in claim 1, wherein said second amplification means includes,.
Primers for the second amplification were biotinylated at their 5 ' ends to facilitate downstream purification steps.
In one example, 14 PCR cycles are used in the second amplification reaction.
Preferably, the second amplification reaction is performed using forward-internal and reverse primers.
The resulting voltage signal is output to the first and second amplification circuits 15A and 15B.
A summary of this second amplification experiment is presented in Table 4.
The Q ' - C ' extension product is displaced by extension of the second amplification primer.
In a preferred embodiment, each second amplification module has at least three possible gain ranges, wherein.
The control circuit 17 stores the output signals from the first or second amplification circuits 15A and 15B.
The source of the second amplification transistor 15 is connected to the ground 3.
Second amplification stage AS, and.
The device of claim 10, wherein each second amplification module comprises,.
At a step 620, a second amplification reaction is performed using a universal primer pair.
In a preferred embodiment of the invention, the length of the second amplification oligonucleotide is 19-40 nucleotides.
The second amplification system is the ligase amplification reaction ( LAR ).
Also included in the kit is a second amplification oligonucleotide having a length of up to 100 nucleotides.
This is the second amplification step, where a single PDE hydrolyses about 1000 cGMP molecules.
The sources of the first and second amplification transistors 19 and 20 are connected to the ground.
Either the first or second amplification oligonucleotide, or both, may be a promoter-primer.
In some embodiments, the second amplification primer comprises sequence C, or a portion thereof, at the 3 ' end.
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