Examples of 'selection agent' in a sentence
Meaning of "selection agent"
A selection agent is a substance or factor used to select for specific organisms or cells with particular traits in a sample. It is commonly used in laboratory settings to facilitate the growth or identification of desired microorganisms
How to use "selection agent" in a sentence
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selection agent
No selection agent should be added during transient transfections.
Thereafter a fast reduction of the selection agent is performed.
Normally no selection agent presents during this step.
Said whole plant displays a tolerance to said selection agent.
The selection agent can be an antibiotic.
The cells are then isolated using a positive selection agent.
Without selection agent is intended to mean without an effective amount of the selection agent.
Thereby one outlier in triplicate was found by comparing AqP of both selection agent conditions.
Shoots resistant to the selection agent are appearing and once rooted are transferred to soil.
Selection is complete when the nodule tissue shows vigorous growth on the selection agent.
One herbicide which constitutes a desirable selection agent is the broad spectrum herbicide bialaphos.
Preferably the second nucleotide sequence encodes a dihydrofolate reductase polypeptide and the selection agent is methotrexate.
The selection agent Herbiace allowed the growth of the transformed cells.
High correlative behavior of stability of cell lines with and without selection agent was determined.
In vitro Selection agent.
See also
Explants are transferred to the same medium containing the appropriate selection agent every three weeks.
Subsequently adding a selection agent to the culture medium ;.
In certain exemplary embodiments, zeocin is used as the selection agent.
In that process, no selection agent is used.
A selection agent for determining a viewing position and a viewing angle ;.
Undesirable recombinants could subsequently be eliminated by applying the selection agent to the microspore medium.
For example, MTX is a selection agent useful for the amplification of the DHFR gene.
Optionally, the concentration of the selection agent is varied.
The selection agent used in conjunction with a DHFR gene is methotrexate ( Mtx ).
Amplification is achieved using a selection agent e . g.
This selection agent can be used with any otherwise suitable host, e.g. ATCC No.
In one embodiment, the concentration of the selection agent is varied.
The fermentation broth further comprised kanamycin monosulfate ( an aminoglycoside antibiotic ) as selection agent.
According to one embodiment, selection involves the use of a selection agent that induces chromosome breakages.
After bombardment, embryos were transferred to callus initiation medium containing glufosinate as the selection agent.
The method of claim 1 wherein the selection agent is the herbicide Bialaphos.
After cocultivation, the leaf explants were cultured on ms medium + timentin and ppt selection agent.
Amplification is achieved using a selection agent e.g. methotrexate ( MTX ) which is inactivated by DHFR.
After one week, original medium was replaced with fresh medium containing selection agent.
Amplification is achieved using a selection agent e.g. methotrexate ( MTX ) which inactivates DHFR.
Embryogenic callus is subcultured every 2 weeks to fresh maintenance medium containing an appropriate selection agent.
One or more antifoam agent ( s ) or selection agent ( s ) may be used.
Resistant colonies that arise after a few weeks are transferred to IMS + selection agent.
In one example, the concentration of the selection agent is varied.
A process acoording to Claim 1, wherein said plant embryos are exposed to said selection agent.
High RFP expression was confirmed in the absence of a selection agent with 10 passages.
Synthetic PPT, the active ingredient in the herbicide Liberty also is effective as a selection agent.
A process according to any of Claims 1 to 9, wherein said selection agent is a cytotoxic chemical.
In these methods the antifolate methotrexate ( MTX ) is used as selection agent.
Typically, a selectable marker gene will confer resistance to a selection agent such as a drug, e . g.
In the case of a selectable gene that encodes hygromycin phosphotransferase, a suitable selection agent is hygromycin.
Table 20, Calculated means of delta SPR with and without selection agent.
Puromycin ( 1 µg / ml ) was added to the medium as a selection agent.
Prokaryotic selection markers include, e.g. the beta-lactamase gene ( conferring resistance to the selection agent ampicillin ).
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