Examples of 'selection cassette' in a sentence
Meaning of "selection cassette"
selection cassette: A selection cassette is a DNA sequence that allows for the selection of cells that have successfully taken up a desired piece of DNA during genetic engineering or gene editing processes
How to use "selection cassette" in a sentence
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selection cassette
The selection marker can be contained in a selection cassette.
The selection cassette may be removed by methods known by the skilled artisan.
The donor comprises up and down joiner oligos and a selection cassette.
The fusión of the selection cassette to downstream exons inactivates the target gene.
This removal is obtained by intragenic recombination between the homologous sequences surrounding the selection cassette.
A selection cassette may be located anywhere in the construct outside the coding region.
The recombinant bacmid can be selected using standard techniques depending on the bipartite selection cassette.
The DHFR selection cassette makes it possible to carry out selection for pyrimethamine.
The restriction sites used to insert the selection cassette are marked as RS.
It contains a selection cassette with the gene she ble and a MCS flanking the fcpA promoter.
In other embodiments, a gBlock can comprise a selection cassette.
In one aspect, the selection cassette is a neomycin cassette.
In some cases, the gBlock does not comprise a selection cassette.
Optionally, the selection cassette is retained in the mice.
In one embodiment, the insert nucleic acid comprises a selection cassette.
See also
In one embodiment, the selection cassette is a neomycin cassette.
In some such methods, the gBlock does not comprise a selection cassette.
Subsequently, the gentamycin selection cassette was removed by FLP-recombination.
In some embodiments, the insert nucleic acid comprises a self-deleting selection cassette.
The bipartite selection cassette comprises 2 different selectable markers.
In some embodiments, the targeting vector comprises a selection cassette flanked by recombination sites.
Optionally, the selection cassette can comprise a drug resistance gene.
In one embodiment, the LTVEC comprises a selection cassette or a reporter gene.
Neo counter selection cassette was amplified using plasmid pSC101 rpsL - neo ( Genebridges ) as template.
The primers were designed in such a way that the pBELOBAC11 Chloramphenicol selection cassette was deleted.
In some methods, the selection cassette comprises a drug resistance gene.
B, Modified allele after FLP mediated deletion of the selection cassette.
As mentioned, the bipartite selection cassette is flanked by LoxP recombination sequences.
In one embodiment, the modified allele comprises a drug selection cassette ( DSC ).
In a second step, the rpsL-neo selection cassette was exchanged with a new sequence.
The linkers had 40 bp overlap to mBAC and 40 bp overlap to a selection cassette.
In another embodiment, the selection cassette is located within the human DNA fragment.
SDC NEO, self-deleting neomycin selection cassette.
In one embodiment, the selection cassette is a hygromycin-TK cassette.
B, Modified allele after FLP mediated deletion of the selection cassette which restores gene function.
In one aspect, the selection cassette is a hygromycin-TK cassette.
Plaque-purified virus MVA-BN-PRO lacking the selection cassette was prepared.
In one aspect, the selection cassette is a Frt'ed hygromycin cassette.
Spec, spectinomycin selection cassette.
In one embodiment, the selection cassette is a Frt ′ ed hygromycin cassette.
Hyg, hygromycin selection cassette.
In one embodiment, the selection cassette is a Frt ′ ed Hyg-TK cassette.
In one embodiment, the selection cassette is a Frt ′ ed neomycin cassette.
In one embodiment, the selection cassette is a Frt ′ ed hygromycin-TK cassette.
For selection purposes, a selection cassette ( floxed for removal by Cre ) was included.
In another disclosure, the selection cassette is located within the a2-a3 intron.
In another embodiment, the selection cassette is located within the a2-a3 intron.
In one aspect, the selection cassette is a Frt'ed Hyg-TK cassette.
A Notl fragment containing the URA3 selection cassette was ligated into Notl-cut pHJJ75.
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