Examples of 'selection marker gene' in a sentence
Meaning of "selection marker gene"
selection marker gene: This refers to a gene that is used in biotechnology to identify and select cells that have been successfully transformed with a desired gene
How to use "selection marker gene" in a sentence
Basic
Advanced
selection marker gene
This allows the second selection marker gene to be transcribed.
The transformed cells are selected using a selection marker gene.
A general example of a selection marker gene is an antibiotic resistance gene.
This does not influence the ability to delete the selection marker gene.
It is to be understood that a selection marker gene may also be native to a fungus.
Dihydrofolate reductase or ampicillin resistance gene may be used as a selection marker gene.
It is to be understood that a selection marker gene may also be native to a plant.
The selection marker gene thus replaces the deleted sigG gene region.
The invention further discloses selection marker gene free recombinant filamentous fungi.
The exogeneous nucleic acid may also comprises at least one selection marker gene.
The invention discloses selection marker gene free recombinant microorganisms.
Selected cells will be recombinant and will have deleted the selection marker gene.
A preferred selection marker gene is the ble gene encoding a protein conferring resistance to phleomycin.
The use of a dominant and bi-directional selection marker gene is preferred.
A selection marker gene is ligated upstream of the IRES sequence.
See also
This type of vector comprises a selection marker gene flanked by DNA repeats as well.
The selection marker gene can be used on an episomal DNA vector.
This plasmid preferably contains an ampicillin-resistance gene as selection marker gene.
The construction of a selection marker gene DNA fragment can be achieved as directed below.
Furthermore, the vector may additionally contain a replication origin or a selection marker gene.
The selection marker gene is preferably flanked by site-specific recombination sequences.
A method according to claim 8 wherein said selection marker gene is a negative selection marker.
However, the selection marker gene in the present invention is not limited to these genes.
The selection marker comprises a non-attenuated selection marker gene operably linked to a promoter ;.
Examples of the selection marker gene are antibiotic-resistant gene and auxotrophic gene.
Strain according to claim 1, wherein said strain further comprises at least one selection marker gene.
A method for obtaining a selection marker gene free recombinant filamentous fungus comprising the following steps,.
The DNA according to claim 2, wherein said other gene is a selection marker gene.
A selection marker gene DNA fragment can be constructed as follows,.
The DNA according to claim 3, wherein the selection marker gene is a chloramphenicol acetyltransferase gene.
The cells may also contain a STOP site, and an additional selection marker gene.
Advantageously, the selection marker gene is selected amongst,.
It also contains the G418 resistance gene as a yeast selection marker gene.
Upon RTS 1 excision, the second selection marker gene is expressed, allowing selection for disimmortalized cells.
A filamentous fungus according to claims 1, 2, or 3 wherein said selection marker gene is an acetamidase gene.
A ccdB selection marker gene from E. coli ;.
A vector according to claim 1 or 2, wherein the selection marker gene is an acetamidase gene.
The B-lactamase selection marker gene is denoted as “ bla ”.
A vector according to claims 29 or 30 wherein the selection marker gene is an acetamidase gene.
You'll also be interested in:
Examples of using Marker
Show more
Spruce marker technologies for sustainable forestry
This is a rare genetic marker known as prognathism
Side marker lights and auxiliary indicator
Examples of using Selection
Show more
Selection of display in list or tile form here
The print reserve selection screen is displayed
Selection of basal suckers ready for planting
Examples of using Gene
Show more
Gene will never be able to digest them
The baldness gene was cause for dread
Gene mckinney pleaded the fifth amendment
