Examples of 'selection medium' in a sentence
Meaning of "selection medium"
selection medium: the environment or platform through which choices or decisions are made or presented
How to use "selection medium" in a sentence
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selection medium
The selection medium lacks hypoxanthine and thymidine.
Microorganisms can be kept in selection medium after selection.
The selection medium may also contain acetosyringone.
Cells were grown in drug selection medium.
Selection medium war replaced two times per week.
Transformants can be selected on selection medium.
The selection medium was renewed after a week or when needed.
An aliquot of untransformed protoplasts was added to selection medium as a control.
The selection medium typically includes streptomycin and kanamycin.
Using an appropriate selection medium.
Selection medium was refreshed twice weekly in the remaining transfected dishes.
Cells containing the construct may be selected by employing a selection medium.
The selection marker together with a suitable selection medium maintains the cloning vector in the cells.
Transductants are selected that are kanamycin resistant by growing colonies on an appropriate selection medium.
The tissues were transferred to fresh selection medium every three weeks.
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Stable transformants are selected for by culturing and regenerating plants on appropriate selection medium.
The selection medium used for cultivation may comprise selection agents for all selectable markers used.
The cells were fed twice a week using the selection medium.
The fused cells are cultured in HAT selection medium containing FCS to screen hybridoma.
The callus tissues were amplified over a period of several months on a selection medium.
Two additional feedings with selection medium were done at 3 day intervals.
Over the next several months callus tissues were amplified on selection medium.
The selection medium was changed every 5 days until colonies appeared.
The cells were cultured in this primary selection medium for four days.
Frequently, a selection medium includes a toxin or other selecting factor that kills non-transformed cells.
The collected cells were expanded by cultivation in selection medium for several days.
This selection medium was refreshed every 3 to 4 days.
The colonies which then form are subcultured several times on the selection medium.
The embryos were transferred to fresh selection medium at two-week intervals.
Initial differentiation experiments were performed with the PPARδ cell lines in the selection medium.
A respective selection step usually involves a selection medium comprising a DHFR inhibitor.
About 20 discrete colonies were randomly picked and bulked further on a fresh selection medium.
The next day, an equal volume of selection medium was added to each well.
Explants can be cultured for about two to four weeks on the selection medium.
Preferably, the cells are cultured in selection medium during the period of time.
Bialaphos-resistant calli were maintained and expanded on selection medium.
The selection medium was changed by new one every 48 h.
In some cases these same mutants appeared on the selection medium more than one time.
Fresh selection medium was provided 3 days, and again 5 days after selection was begun.
The Agrobacterium is grown in appropriate selection medium.
Transgenic lines were recovered on selection medium supplemented with 100mg/l of spectinomycin.
Selection of fused cells was carried out in HAT selection medium.
Transgenic lines were recovered on selection medium supplemented with 50mg/l of kanamycin.
Plates were fed every 4 days with the appropriate selection medium.
Transgenic tobacco plants were regenerated on selection medium supplemented with 300 mg / l of kanamycin.
These cells were trypsinized, and then combined together using the selection medium.
Thereafter, the transformed explants were cultured in selection medium to select transgenic callus see FIG.
Resistant clones were separately isolated and cultured 14 days after applying the selection medium.
The tissue was subcultured by transferring to fresh selection medium for a total of about 2 months.
For Agrobacterium - mediated transformation, an antibiotic to eliminate Agrobacterium is included in the selection medium.
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Examples of using Medium
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