Examples of 'shuttle vector' in a sentence
Meaning of "shuttle vector"
shuttle vector: In genetics and biotechnology, a shuttle vector is a type of DNA molecule that can replicate in multiple host organisms. This phrase is commonly used in the context of genetic engineering and gene transfer between different species or cell types
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- A vector constructed, usually with selectable markers and tags, so that it can propagate in two different host species.
How to use "shuttle vector" in a sentence
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shuttle vector
Such a shuttle vector includes the followings.
Host strain containing the plasmid shuttle vector.
Such a shuttle vector is exemplified by the followings.
Construction of receptor expression cloning cassette and shuttle vector.
The vector may be a shuttle vector that functions in multiple hosts.
Host strain transformed with the plasmid shuttle vector.
Design of shuttle vector.
The obtained fragments were cloned into the pacCMV shuttle vector.
It may be a shuttle vector capable of gene exchange between different host strains.
The multiple cloning site is another element of the shuttle vector.
A shuttle vector may be used that can exchange a gene between host strains.
The two fragments were combined in a shuttle vector and subsequently cloned into the phage genome.
The resulting PCR fragment is purified and digested and cloned into the shuttle vector.
Structure of proviral shuttle vector and the predicted structure of rAAV circular intermediate monomers.
It is the recombinant baculovirus containing only the shuttle vector with no CEA insert.
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An advanced shuttle vector system has been developed by Davidson et al.
In certain embodiments, the vector is a shuttle vector.
Thus preferably the retroviral shuttle vector of the present invention are SIN vectors.
The shuttle vector according to any preceding claim further comprising DNA transposable elements defining a transposon.
The cDNA were then inserted into a pREP3Y shuttle vector.
Purifying the baculovirus shuttle vector containing the transposable element ;.
This entire sequence is desirably employed as the 3 ' sequence of an pAdA shuttle vector.
Fig.3 show a flow of construction of shuttle vector carrying lacZ as a reporter gene.
The mer operons of these plasmids are inserted separately in the shuttle vector pRR3.
This strain was transformed with an integrative shuttle vector carrying a firefly luciferase ( lux ) expression cassette.
In other embodiments, the foreign nucleotide sequence is at least a part of a one-way shuttle vector.
The generation of this shuttle vector is shown in Figure 2.
Construction of Escherichia coli - bifidobacterium shuttle vector.
This ensures that the said shuttle vector must replicate when transformed into I . bulgaricus.
Removal of the C. perfringens origin of replication converts the shuttle vector into a suicide vector.
The resultant shuttle vector confers nourseothricin resistance to S. cerevisiae.
The DNA plasmid may be capable of functioning as a shuttle vector between A . succinogenes and E. coli.
This shuttle vector replicates both in staphylococci and E. coli.
The vector used is a Gram positive / E. coli shuttle vector that has a medium copy number in B.
A shuttle vector for transforming insect cells, comprising,.
The studies utilized a bifunctional shuttle vector capable of replicating in both E. coli and Bacillus spp.
Construction of dual-origin temperature-sensitive shuttle vector.
Approximately 5 µg of the shuttle vector pWHM3 was digested with Sph I and Bam HI.
The obtained recombinant plasmid was designated as shuttle vector pJW241.
With the constructed shuttle vector pVK7, aspC was ligated.
The insert from these clones was subcloned into shuttle vector pSL401.
A shuttle vector comprising,.
A bacterium comprising the shuttle vector of claim 12.
The shuttle vector of claim 11, further comprising an inducible transposase gene within the transposon.
Plasmid pHT01 is a Bacillus-E . coli shuttle vector that replicates vía a theta mechanism.
The shuttle vector of claim 11, wherein the selectable marker gene is within the transposon.
The insert from these clones was subcloned into shuttle vector pSL40I.
Insect cells transformed with the shuttle vector according to any one of claims 1 to 10.
The shuttle plasmid was denominated pChAd30 EGFP shuttle vector.
This generates pBr/Ad35 . PRAfib, a shuttle vector suitable to introduce fiber sequences of alternative serotypes.
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