Examples of 'single primer' in a sentence
Meaning of "single primer"
single primer: Refers to a specific type of starting material used in processes such as DNA amplification or PCR (Polymerase Chain Reaction). It indicates the use of only one primer molecule in a reaction
How to use "single primer" in a sentence
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single primer
Nucleic acid amplification using single primer.
Such an approach to single primer amplification is described above.
Single primer extension abr.
This method is referred to as single primer amplification.
The single primer amplifies both templates and exponential amplification follows.
This is an example where a single primer acts as a pair.
After extension of the primer the polynucleotide can be amplified using a single primer.
The method find use in single primer amplification.
The above single primer amplification product was next purified and sequenced as described below.
Method producing a polynucleotide for use in single primer amplification.
In principle, a single primer is sufficient for generating band patterns.
There is no requirement to use a thermostable polymerase for a single primer extension reaction.
As a result single primer amplification occurs, rather than a PCR amplification.
Another advantage of the methods of the invention is that only a single primer is required.
This demonstrated the ability of the single primer amplification to amplify a single species.
See also
Another advantage of the methods of the disclosure is that only a single primer is required.
The use of the present method in single primer amplification is depicted in Fig.
The target nucleic acid sequence is exponentially amplified through a process that involves only a single primer.
The linear growth in copy number with a single primer was confirmed by Kim et al.
MLPA facilitates the amplification and detection of multiple targets with a single primer pair.
In one embodiment, a single primer pair is used.
Tandemly linked copies of a closed circle DNA molecule may be formed by using a single primer.
Step 1 shows binding a single primer to a target RNA.
The ss-slDNA of the invention can be amplified with a single primer.
Within a single Primer ID, when three or more sequences were present, a consensus sequence was formed.
When the repetitive sequence is Drrep only a single primer is used.
The product of single primer amplification is detected, not the product of PCR amplification.
These were not expected to have amplifiable DNA by single primer amplification.
The single primer PCR product is analysed and extracted from 1 % agarose gel if necessary.
The method finds particular application, for example, in single primer amplification assays.
Single primer PCR is carried out using a DNA polymerase according to manufacturer 's instructions.
The ss-slDNA produced by the method of the invention can be amplified with a single primer.
Single Primer Isothermal Amplification ( SPLA ) also involves multiple polymerases and RNaseH.
Preferably, the PCR is performed on a single strand with a single primer pair.
Fusarium verticillioides, fumonisins, single primer amplification reaction, mating types.
For example, the cDNA samples can be amplified by single primer PCR.
SNP, CAPS marker, single primer extension.
Another use is the application of the polymerase chain reaction ( PCR ) using a single primer.
For DNA, polymerase chain reaction ( PCR ) and single primer amplification are convenient.
Thus, many sequences ( up to 40 ) can be amplified and quantified using just a single primer pair.
This results in a pool of SISPA ( sequence-independent single primer amplification ) - amplified DNA fragments . Example 8.
The adapter-target constructs can be amplified by PCR using this single primer species.
The PCR is multi-template PCR ; only a single primer pair is used.
Both target nucleic acid sequences were simultaneously amplified using a single primer pair, KY78 and KY80.
The amplification of the PCVII 412 viral DNA by single primer PCR was successful.
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