Examples of 'site directed mutagenesis' in a sentence
Meaning of "site directed mutagenesis"
Site-directed mutagenesis: A molecular biology technique used to make specific and intentional changes in the DNA sequence of a gene. This can involve adding, deleting, or substituting nucleotides to study the effects of these changes on gene expression and protein function
How to use "site directed mutagenesis" in a sentence
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site directed mutagenesis
This would be achieved by site directed mutagenesis.
Site directed mutagenesis was performed on the arch region.
The mutants were obtained by site directed mutagenesis.
Site directed mutagenesis.
Construction of vector for site directed mutagenesis.
By site directed mutagenesis the two amino acids were substituted separately.
This is accomplished by conventional methods of site directed mutagenesis.
In vitro site directed mutagenesis allows to exchange desired nucleotides within a plasmid backbone.
Such alteration may be accomplished by site directed mutagenesis.
Subsequent site directed mutagenesis may be used to alter additional nucleotides in any mutant.
The nucleotide sequence can be modified by site directed mutagenesis.
Site directed mutagenesis in these helical regions could block the ability to dimerize.
Galactose positive mutants can also be generated by site directed mutagenesis.
Site directed mutagenesis on these sites inactive the enzyme both in vivo and in vitro.
Such plant can also be obtained by in situ site directed mutagenesis.
See also
Site directed mutagenesis of nucleic acids and subsequent expression of the proteins is another route.
Secondly it may be achieved by site directed mutagenesis of the same coding region.
This will facilitate manipulation of cellular lifespan through site directed mutagenesis.
Describes the use of site directed mutagenesis for the purpose of reducing IgE binding.
The modification was introduced at the DNA level using site directed mutagenesis.
Site directed mutagenesis may then be achieved using specific mismatched synthetic oligonucleotides by standard methods.
Mutations were made using standard site directed mutagenesis techniques with the appropriate mutagenic primers.
Site directed mutagenesis was as described in Kramer et al ref.
This can be done by site directed mutagenesis or PCR.
Modifications to the DNA sequences can be made by using site directed mutagenesis.
Other site directed mutagenesis techniques can also be employed with CKI nucleotide sequences.
Methods of altering the DNA sequences include but are not limited to site directed mutagenesis.
Site directed mutagenesis was performed by PCR using primers containing the desired nucleotide changes.
Appropriate DNA inserts can also be obtained which code for polypeptide having site directed mutagenesis.
The process of site directed mutagenesis follows the instruction of the kit from Strategene Corp.
Substitution and insertion mutations were prepared using the Amersham Site directed Mutagenesis Kit.
Site directed mutagenesis Site directed mutagenesis was performed by PCR.
In a preferred embodiment, site directed mutagenesis is used.
Oligonucleotide site directed mutagenesis was performed using the mutagenesis kit supplied by Amersham International PLC.
In the examples that follow, site directed mutagenesis was used.
Minor alterations to an enzyme ( and its catalytic properties ) are possible vÃa site directed mutagenesis.
Alternatively, site directed mutagenesis techniques may be used.
In that event, the restriction sites may be introduced by site directed mutagenesis.
Techniques in site directed mutagenesis are well-known in the art.
N-terminal glutamine was therefore substituted with asparagine using site directed mutagenesis.
Specifically, site directed mutagenesis is used to graft the CDRs onto the framework.
From the original packaging plasmid, site directed mutagenesis was performed.
For example, site directed mutagenesis can be used to modify a reovirus nucleic acid sequence.
Alternatively, such polynucleotides may be obtained by site directed mutagenesis of characterised sequences.
Site directed mutagenesis was performed based on 3D structure analysis ofPLP-binding polypeptides.
Alternatively, such polynucleotides may be obtained by site directed mutagenesis of characterized sequences.
Site directed mutagenesis was performed employing standard procedures using the Quickchange mutagenesis kit ( Stratagene ).
The derivatization can, for example, be carried out by means of site directed mutagenesis.
Primers used for site directed mutagenesis are listed in Table 2 below.
Any convenient method for introducing mutations may be employed, including site directed mutagenesis.
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All feedback should be directed by mail or in person to
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Examples of using Mutagenesis
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Mutagenesis kit according to the supplierʼs instructions
Other forms of chemical mutagenesis are also available
Mutagenesis may be performed by any suitable method
