Examples of 'sodium phosphate buffer' in a sentence
Meaning of "sodium phosphate buffer"
sodium phosphate buffer: In chemistry and biology, a sodium phosphate buffer is a solution containing a mixture of sodium phosphate salts, used to maintain a stable pH level in a system or to prevent drastic changes in acidity or alkalinity
How to use "sodium phosphate buffer" in a sentence
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sodium phosphate buffer
Typically a sodium phosphate buffer is used.
In a further embodiment the elution buffer is a sodium phosphate buffer.
As the pH adjuster, sodium phosphate buffer and sodium carbonate buffer are suitably used.
The preferred buffer for use herein is sodium phosphate buffer.
The PEG-aIFN in sodium phosphate buffer was loaded onto a QHD anion exchange column.
Diafiltration was accomplished using sodium phosphate buffer at neutral pH.
Following low speed centrifugation the PEG-precipitate was resuspended in approximately 1/10 volume of sodium phosphate buffer.
The precipitate can dissolved in sodium phosphate buffer and centrifuged.
In some embodiments, equilibration may take place in a solution comprising a Tris or a sodium phosphate buffer.
The resulting pellet was resuspended in a sodium phosphate buffer containing sodium chloride.
The process of Claim 13 wherein the aqueous solution comprises ammonium chloride or sodium phosphate buffer.
The proteins were more soluble in sodium phosphate buffer than in deionized water.
The buffering agent to be used in the present solution is a sodium phosphate buffer.
In the present invention, the buffer is sodium phosphate buffer having a pH ranging from 3 to 8.
The beads were resuspended in the original volume using the sodium phosphate buffer.
See also
A 10 mM sodium phosphate buffer pH 7.5 was also added up to the final working volume.
The preferred buffer for use herein is a sodium phosphate buffer system.
Sodium phosphate buffer pH6.8 was added to the polysaccharide solution with a final concentration of 25 mM.
Preference is generally given to the use of a sodium phosphate buffer for buffering the medium.
The column was then reequilibrated with 5 ml of the sodium phosphate buffer.
In another embodiment, the sodium phosphate buffer of step ( d ) is 25 mM sodium phosphate.
Donor formulations buffered to pH 5 with sodium phosphate buffer.
At least 50 mM sodium phosphate buffer is needed to dissolve up to 20 mM HQS.
Preferably, the buffer is sodium phosphate buffer.
The composition of claim 1, wherein the phosphate buffer is a sodium phosphate buffer.
The bacterial pellets are taken up in sodium phosphate buffer 50 mM, pH 7.0 and centrifuged again.
The concentrate is passed through a column pre-equilibrated with sodium phosphate buffer.
The solution was diluted to 4000 g with sodium phosphate buffer and stirred for five minutes.
A similar experiment was carried out with DMSO stock diluted in 10mM sodium phosphate buffer.
Cells were recovered, washed with sodium phosphate buffer ( PBS ) and sedimented.
This dye solution can be adjusted to pH 4.5 by adding 5 parts of a sodium phosphate buffer.
Beads were then transferred to 50 mM pH 7 sodium phosphate buffer containing 50 mM acrylonitrile.
In a preferred embodiment, the composition comprises about 10 mM dibasic sodium phosphate buffer.
A formulation comprising a chondroitinase in a sodium phosphate buffer at a concentration of about 750 to 1000 mM.
In another embodiment, the phosphate buffer is 10 mM dibasic sodium phosphate buffer.
The mobile phase buffer was 10 mM sodium phosphate buffer supplemented with 20 % ethanol by volume.
Phosphate buffers are preferred, particularly sodium phosphate buffer.
The eluent was 67 mM sodium phosphate buffer pH 7.4 with different amounts of BCD.
The column is equilibrated with a 10 mM sodium phosphate buffer.
PSA was eluted using 8mM sodium phosphate buffer pH 7.2 and sodium chloride.
In a further embodiment, the phosphate buffer is 10 mM dibasic sodium phosphate buffer.
Blocking was achieved by sodium phosphate buffer containing bovine serum albumin ( BSA ) ( pH 7.0 washing buffer ).
The reactions were carried out in 50 mM sodium phosphate buffer pH 7.
Briefly, one volume of 0.3 M Sodium phosphate buffer was added to 9 volumes of Influenza virus preparation.
The supernatant was discarded, the pellet was washed 3 times in 20 mOsM sodium phosphate buffer.
The eluate was neutralised with 0.5 M sodium phosphate buffer pH 6.8 and the phage plated for analysis.
However, additional suitable metal salt ( s ) may be added to the sodium phosphate buffer.
Unadsorbed proteins were washed out with 50 mM sodium phosphate buffer containing 1 M NaCl.
The haemoglobin, concentrated on the filter was washed twice with 50 mM sodium phosphate buffer.
Said composition has a high ionic strength corresponding to a sodium phosphate buffer with a concentration of 150 mM.
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Examples of using Phosphate
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