Examples of 'southern blotting' in a sentence
Meaning of "southern blotting"
southern blotting: Southern blotting is a laboratory technique used to detect specific DNA sequences in a DNA sample. It involves gel electrophoresis to separate DNA fragments by size and transfer them to a membrane for analysis
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- present participle of Southern blot
How to use "southern blotting" in a sentence
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southern blotting
Southern blotting revealed a single copy of the gene.
Another possibility for effecting the identification is by means of southern blotting.
Southern blotting and hybridization.
Clones are isolated and analyzed by Southern blotting.
Southern blotting is one example where this method is used.
Virus particle concentrations are based on Southern blotting.
Southern blotting is still useful in the diagnosis of disorders caused by trinucleotide repeats.
These fractions are then analyzed by Southern blotting.
Southern blotting confirmed that the clonal lines were indeed uniform and transformed with the vector.
The expansions are easily detected in Southern blotting assay.
Nonradioactive Southern blotting procedures were conducted as follows.
Genome integrity was confirmed by Southern blotting.
Detection may be by Southern blotting and hybridization with a labeled probe.
The obtained positive clone was analyzed by Southern blotting.
RFLP analysis involves southern blotting and other techniques.
See also
The number of sites of integration was also determined by Southern blotting.
Southern blotting was made to locate a specific sequence of DNA within a complicated mixture.
Probing may employ the standard Southern blotting technique.
Southern blotting is designed to locate a particular sequence of DNA within a complex mixture.
The copy number of integrated genes was determined by Southern blotting.
Seven rearrangements were detected by Southern blotting but precise allele identification was not possible.
The positive clones were purified to homogeneity and analysed by Southern blotting.
Southern blotting was then used to assess the stability of the transformed DNA sequences.
Combinations with further techniques such as Southern blotting are also envisaged.
Southern blotting for DNA hybridisations.
Hybridization and washings were as described for the Southern blotting analysis.
Random integrations were excluded by Southern blotting with a neomycin DNA probe and internal probes.
The gels are transferred onto nitrocellulose using standard Southern blotting techniques.
For a review of Southern blotting see Davis et at.
The final chromosomal configuration is determined by restriction analysis and Southern blotting.
For a review of Southern blotting see Davis et al.
The purified defective poxviruses are grown to larger scale and examined by Southern blotting.
Southern blotting analysis of genome DNA.
A conventional gel electrophoresis method followed by a Southern blotting can also be used.
Screening was done by Southern blotting of DNA obtained from tail tissue.
The number and stability of the integrated expression cassettes is determined by Southern blotting.
The cDNA is then transferred by Southern blotting to Zetabind membrane.
The episomal persistence of the PyV origin containing plasmids was analyzed by Southern blotting.
The general procedure for Southern blotting was taken from Maniatis et al.
The chromosomal structure of two subclones derived from two clones was analyzed by Southern blotting.
Southern blotting analysis Further confirmation was carried out by Southern blotting.
The purified defective viruses are then grown to larger scale and examined by Southern blotting.
The mutant genotype is confirmed by Southern blotting or PCR demonstrating the appropriate deletion.
Transmission of the targeted loci was confirmed by Southern blotting and PCR.
Results of PCR and Southern blotting are shown in FIG.
The number of amplified EPO cDNA copies is detected by Southern blotting.
Analysis by PCR and Southern blotting Plastid transformants were identified by PCR amplification.
Deletion was validated with Southern blotting.
Southern blotting analysis are carried out as described in example 1 and in standard protocols.
Transformants were characterized using PCR and Southern blotting techniques.
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Examples of using Blotting
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This blotting paper absorbs ink well
Saturating kraft behaves like blotting paper
You are blotting oil off your face
Examples of using Southern
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In southern accent just like ringing a bell
That is the way southern people talk
The southern group has just been released
