Examples of 'specific primers' in a sentence
Meaning of "specific primers"
Specific primers are short DNA sequences used in PCR (Polymerase Chain Reaction) to amplify a specific target DNA sequence. They are designed to anneal to complementary regions on the DNA strand, allowing DNA polymerase to synthesize new DNA strands that are complementary to the target sequence
How to use "specific primers" in a sentence
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Advanced
specific primers
Allele specific primers can also be used.
No restriction enzyme digestion or specific primers are required.
The target specific primers contain a universal tail.
These were paired up with insert specific primers.
Several specific primers were also synthesized for this purpose.
Technologies with subtype specific primers and probes.
Specific primers were designed for selective amplification of full length cDNA.
Genes amplification were performed using specific primers and conditions.
Using specific primers the cDNA was amplified for further cloning.
A disadvantage of this method is generating the sequence specific primers.
Selected BACs were retested using specific primers for the candidate gene.
Such amplification methods typically use one or more specific primers.
Specific primers for use with this gene are given in this paper.
And gaps between the contigs were closed by using specific primers.
Specific primers for the amplification of the coding regions can be constructed.
See also
Specific RNAs are amplified using message specific primers or random primers.
Gene specific primers are then used to pull down specific gene fragments of interest.
Multiplex reactions are accomplished by careful selection and optimization of specific primers.
The mixture of target specific primers includes hundreds of targets per reactions.
The amplification reaction may be performed using one or more specific primers.
According to the invention specific primers and probes are used in said analysis method.
Specific primers are used to amplify variable heavy and light chain domain nucleic acids.
Accordingly it is possible to construct relatively specific primers flanking active site loops.
Specific primers were synthesized to amplify the putative autoantigen gene from the chondrocyte cDNA.
The amplification reactions using the specific primers are carried out in separate reaction vessels.
All of the aspecific amplification products undergo a new specific amplification using specific primers.
The two allele specific primers carry a tag of unique sequences that determine each allele.
Two nested PCRs are usually performed using two nested known sequence specific primers.
The use of specific primers and fluorescent labels can distinguish between wild type and mutations.
Parallel sequencing of different genes may also be achieved by amplification using gene specific primers.
Sequencing was performed using specific primers on double stranded DNA.
Specific primers or probes hybridized only to the target DNA.
PCR amplification requires the use of specific primers.
The specific primers are able to amplify a DNA fragment of approx.
A PCR amplified product was generated using appropriate specific primers.
PCR needs specific primers that indicate what needs to be multiplied.
Correct cloning was confirmed by PCR and sequencing using specific primers.
Specific primers are designed enabling PCR amplification of the complete virD loci.
The only changes were in the Coix specific primers that were used.
Some specific primers for the PCR reaction are suggested in the experimental part.
Primer extension is done with allele specific primers and a DNA polymerase.
Specific primers for the control sequence are then added to the PCR reactions.
Isolated DNA fragments were further amplified using adaptor specific primers.
DNA fragments amplified using the specific primers are shown by single lines.
Colonies were screened for the insert by colony PCR using insert specific primers.
The approach of using miRNA specific primers with a small hairpin was developed at ABI.
The resulting first strand cDNA was amplified in using two HGV specific primers.
This may be achieved using specific primers located in the target region of Enterococcus species.
The presence of the gene of interest was confirmed by PCR using gene specific primers.
Other allele and group specific primers and amplification methods are described in the Examples.
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