Examples of 'stable cell line' in a sentence
Meaning of "stable cell line"
stable cell line: This phrase is commonly used in biology and biotechnology to describe a population of cells that exhibit uniform characteristics and can be reproduced consistently over multiple generations. It refers to a group of cells that have been carefully cultivated to maintain specific traits and stability in culture
How to use "stable cell line" in a sentence
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stable cell line
Puromycin resistant stable cell line was selected.
A stable cell line was then generated by zeocin selection.
A cell line can be then isolated to provide a stable cell line.
The stable cell line of the invention can be of human or animal origin.
Selectable ( or selection ) markers are typically utilized within stable cell line development.
Most preferably a stable cell line capable of producing monoclonal antibodies is produced.
Fibroblasts, used as control cells, were obtained from a stable cell line as previously described.
A stable cell line was established to produce a large amount of the chimeric proteins.
Alternately, cells that have been propagated in culture as a stable cell line may be used.
The stable cell line of the invention can originate from human immortalized cells.
The present invention further encompasses recombinant expression of an OsENOD93 protein in a stable cell line.
Final stable cell line selection was based on yield and activity of the expressed protein.
Characterisation of the stable cell line SK6b2.
In addition, stable cell line generation can be performed.
Typically, the cell expressing TLR9 will be from a stable cell line.
See also
Once a stable cell line was established, the line was expanded for use.
Similarly, the cell line which produces the CA8 antibody is a stable cell line.
Stable cell line producing the monoclonal anti-idiotypic antibody according to claim 1.
This assay was developed using a stable cell line expressing IC-GPCR-2, generated as follows.
Stable cell line transfection, process development and generation of a Master Cell Bank is performed.
Sucrose, D-tryptophan, saccharin, and aspartame dose responses are shown for on stable cell line.
A stable cell line transformed by the method of claim 15.
Antibodies were purified from the single stable cell line supernatants using protein A-sepharose ( Pharmacia ).
For stable cell line generation, constructs in pCMV6 were used.
Improved production of a therapeutic transgene-encoding lentiviral vector using a HEK293T . TRIP stable cell line.
The final stable cell line was grown in presence of 3µg/ml of puromycin.
Characterisation of the stable cell line SK6c26.
The S2/CHIK-sE2 stable cell line was obtained by successive passages in presence of blasticidin.
Use as claimed in claim 13 or 14, wherein the eukaryotic host cell is a stable cell line.
A continuous stable cell line which secretes the monoclonal antibody of any one of claims 19 to 21.
Purification of MANF2 protein from Sf9-hMANF2 stable cell line.
The stable cell line expressing FGFR4-directed shRNA showed effective knock-down of FGFR4 protein expression.
MANF2 from Sf9-hMANF2 stable cell line was analyzed equal manner.
A stable cell line was generated transfection of CT26 cells using Lipofectamine ( Gibco BRL ).
Sequences of rescued P-element ends from a stable cell line transformed with the vector pDM79IE-2.
HEK 293 cells were transfected with the proTNFa cDNA ( wild ) to prepare a stable cell line.
PC3TenB2 Medium stable cell line was generated by Genentech.
Stable cell line preparation and assay techniques are adapted from Kingston, A. E., et.
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