Examples of 'sticky ends' in a sentence
Meaning of "sticky ends"
sticky ends: In molecular biology, 'sticky ends' refer to the single-stranded overhangs that result from certain restriction enzyme cleavage sites. These sticky ends are complementary and can base pair with each other, facilitating the joining of DNA fragments
How to use "sticky ends" in a sentence
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sticky ends
The sticky ends or cohesive ends form base pairs.
Tagging can comprise attachment to blunt ends or sticky ends.
The sticky ends can be reattached by another enzyme called ligase.
Exonucleases may cleave the phosphodiester bonds at blunt ends or sticky ends.
This changes the sticky ends available to capture a new pattern component.
The method involves cleaving nucleic acids to form fragments with ambiguous sticky ends.
Blocks have sticky ends which allow you to connect one to another.
They then developed Monte Carlo simulations to include modelling the specific binding between sticky ends.
The sticky ends were filled in with Klenow polymerase.
The fragment was digested with the enzymes Bsa I and Hin d III to generate sticky ends.
The DNA comprises sticky ends after the treatment with the restriction enzyme.
This led to oligo 's with the correct sticky ends.
The sticky ends are filled in a reaction with Klenow DNA polymerase.
In some embodiments, two or more nickases can be employed to generate sticky ends.
The sticky ends are converted to blunt ends by Klenow DNA polymerase.
See also
Several enzymes with a 4-bp recognition sequence are known which produce sticky ends.
The sticky ends of the DNA are filled in a reaction with Klenow DNA polymerase.
In some embodiments, a ligase may ligate nucleic acids having both blunt and sticky ends.
The sticky ends are filled with Klenow polymerase and the DNA is dephosphorylated with CLAP.
The 5 ' ends of the synthetic DNAs contain the sticky ends of the EcoRI restriction site.
Other sticky ends can be used other than an A or T overhang.
Figure 33 depicts staggard cutting of a target nucleic acid using two nickases and generating sticky ends.
The sticky ends of the restriction site are filled in a reaction with Klenow DNA polymerase T.
The linker is ligated to the BglI sticky ends of DNA as described in Example 3.
Sticky ends of plasmid are dephosphorylated using CLAP enzyme, which is inactivated prior ligation.
The PCR product is digested with the appropriate restriction enzyme ( s ) to generate sticky ends.
Alternatively, compatible sticky ends could be produced directed by restriction enzyme digestion of a cDNA clone.
Cpf1 leaves one strand longer than the other, creating sticky ends.
The 5 ' end of the synthetic DNA contains the sticky ends of the EcoRI restriction site.
The sticky ends of the Notl fragment were filled in by means of the T4 polymerase.
Therefore, YEp13 was cleaved with XhoI and SalI and the sticky ends were filed.
Then the sticky ends are filled in with T4 DNA polymerase as below.
The Klenow fragment will fill in at 5 ' sticky ends.
In some embodiments, other sticky ends can be used other than an A or T overhang.
In some embodiments, the cleavage may be staggered, i.e. generating sticky ends.
EcoRI creates 4 nucleotide sticky ends with 5 ' end overhangs of AATT.
The mixture was incubated for 20 h at 14°C if the ligated DNA fragments had sticky ends.
Then, the sticky ends were blunted using DNA Blunting Kit ( Takara Shuzo ).
This cleavage results in sticky ends which are 4 b.p. long.
In this manner oligo 's with the correct sticky ends are created, i.e. KpnI sticky ends.
It creates 4 nucleotide sticky ends with 5 ' end overhangs of AATT.
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