Examples of 'strand synthesis' in a sentence
Meaning of "strand synthesis"
strand synthesis - The process of creating new strands of DNA or RNA molecules by linking nucleotides together in a specific sequence according to the template strand
How to use "strand synthesis" in a sentence
Basic
Advanced
strand synthesis
First strand synthesis was accomplished as described above.
The resulting cDNA was used for second strand synthesis.
Minus strand synthesis continues from this region.
Other techniques used were cDNA first strand synthesis and protein phosphorylation.
First strand synthesis may then performed using a reverse transcriptase.
A second strand can be generated using standard second strand synthesis techniques.
The second strand synthesis also requires a primer.
A sequence specific primer can be introduced into the reaction mix to prime second strand synthesis.
Second strand synthesis was then performed.
One microgram of purified mRNA was applied for a first strand synthesis followed by PCR amplification.
The first strand synthesis reaction can further comprise a thermal cycler.
The step is catalyzed by a DNA polymerase catalyzing complementary strand synthesis which includes strand displacement.
By skipping second strand synthesis scAAV allows for rapid expression in the cell.
The resulting short heteroduplex can serve as primer for second strand synthesis by DNA polymerase.
The product of complementary strand synthesis can be detected by methods known in the art.
See also
The final extension time was 9 min to ensure completion of the strand synthesis.
A kit can comprise a second strand synthesis primer comprising a binding moiety.
Moreover, it is preferable that the primer can be a template for complementary strand synthesis.
Eventually lagging strand synthesis stops and the lagging strand clamp is unloaded.
In the present invention, substrates required for complementary strand synthesis are further added.
The second strand synthesis is carried out under known procedures set forth in the art.
According to the present invention, substrates required for complementary strand synthesis are further added.
The complementary strand synthesis from loop primer R annealed to the structure of Fig.
In the present invention, a mismatch at this location most effectively inhibits complementary strand synthesis.
The second strand synthesis requires a sequence specific primer and Taf DNA polymerase.
A labeled polynucleotide is formed by the incorporation of the labeled deoxynucleotides during polymerase-mediated strand synthesis.
The second strand synthesis requires a sequence specific primer and Tma DNA polymerase.
The following outlines procedures to be used . First strand synthesis.
During the following Second Strand Synthesis the library is converted to dsDNA.
DNA strand synthesis is then initiated starting from the RA primer Fig.
A DNA polymerase-catalyzing complementary strand synthesis which includes strand displacement ;.
Second strand synthesis is with a mixture of Klenow, which contains strand displacement activity.
In Qther words, efficient complementary strand synthesis is not expectable in such cases.
Reverse Transcription was performed with 10 µg total RNA followed by second strand synthesis of cDNA.
Synthesis of cDNA First Strand Synthesis The synthesis of cDNA molecules was accomplished as follows.
Subsequently, the overlapping CDR portions are annealed to each other for complementary strand synthesis reaction.
While leading strand synthesis continues, a new site of lagging stand synthesis is formed.
Iii a DNA polymerase catalyzing complementary strand synthesis which includes a strand displacement ;.
DNA strand synthesis is initiated starting from F1 annealed by the intrachain hydrogen bonds.
DNA polymerase catalyzing complementary strand synthesis which includes strand displacement ;.
Second strand synthesis was carried out using T4 DNA polymerase, first strand cDNA as template.
Once the strand is completed, second strand synthesis is initiated from the viral RNA.
The cDNA was resuspended in 98 µl water for use in second strand synthesis.
In contrast, lagging strand synthesis is accomplished in short Okazaki fragments.
Its 3 ' - side is required to impart a starting point for complementary strand synthesis to those loops.
Figure 7B shows second strand synthesis of the three primer extension products.
Figure 9, Comparison between Silica column and SPRI purification after the 2nd strand synthesis.
After dG tailing, second strand synthesis was performed using C-tailed oligonucleotide primers.
FIG . 34 illustrates an exemplary result after RT-PCR and 2nd strand synthesis process.
After the second strand synthesis the double-stranded cDNA was isolated using protocol R-pellet.
You'll also be interested in:
Examples of using Strand
Show more
Specialized strand for students with disabilities
But there is one different strand here
A strand of hair that has titanium dioxide
Examples of using Synthesis
Show more
Compilation and synthesis of fourth national communications
Two other documents complete this synthesis
Third synthesis report on activities implemented jointly
