Examples of 'subculturing' in a sentence
Meaning of "subculturing"
Subculturing is a term used in microbiology to describe the process of transferring a small sample of cells from a culture to a fresh growth medium to allow for continued growth and study
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- present participle of subculture
- The preparation of a subculture
How to use "subculturing" in a sentence
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subculturing
Subculturing of the positive tubes on a confirmation medium.
This action is called subculturing or passaging the cells.
Subculturing was performed by the following method.
Nutrient dextrose agar for use in subculturing only.
Each round of subculturing is referred to as a passage.
Cloning employed feeder cells as described above for subculturing.
Every cycle of subculturing the cells is known as a passage.
This is called subculturing.
This subculturing process was repeated twice.
Somatic embryos developed into plants after subculturing on a basal meduim.
Subculturing may be carried out in the following manner.
Subculture flasks as per protocol described above for subculturing cells.
Each cycle of subculturing the cells is called a passage.
This is repeated numerous times over several months and is known as subculturing.
Subculturing of the positive tubes.
See also
Purify by subculturing.
Subculturing may be conducted as follows.
The cell line was maintained in flask culture by continued subculturing into fresh media.
This is done by subculturing working stocks of cells before they differentiate.
The traditional protocol for culture maintenance is the serial subculturing of metabollicaly active cultures.
Growing and subculturing stem cells for many months.
AP activity was not measured every time subculturing was performed.
This subculturing procedure was continued until the end of the test period.
TrypLE can be used for subculturing the cells.
Subculturing the isolate to the various media reveals the presence of true hyphae.
No changes were observed in HSA productivity between before and after subculturing.
The protocol for subculturing cells depends heavily on the properties of the cells involved.
The S type did not reappear with subsequent subculturing.
Subculturing and maintenance has been performed as described in the supplementary materials and methods section.
The MFC assay is performed by subculturing the microtiter wells from the MIC test.
Subculturing is continued under the same conditions until the callus is a special type of callus.
These crossing co-cultures were maintained over two years by subculturing.
Their work included subculturing virulent strains of the tuberculosis bacillus and testing different culture media.
Microbial presence may also be assessed by subculturing and / or staining.
The stability in subculturing was further evaluated in terms of HSA yield.
Microbial contamination may also be assessed by subculturing and / or staining.
Subculturing of the Cells.
Cultures are routinely preserved by freeze-drying, and some strains by subculturing.
Preferably, subculturing is conducted at the time of being sub-confluent or confluent.
These qualities are affected by environmental factors and long-term subculturing.
Eight days after subculturing the highest number of PEMs / ml is achieved.
The cultivation on this medium was continued with subculturing at intervals of 3 to 4 weeks.
Three new phenotypes are thus obtained ; two proved to be stable upon subculturing.
The above-mentioned subculturing may be conducted multiple times as needs arise.
Sustainability, refers to a cell culture that can multiply indefinitely by subculturing passages.
If less than the desired amount, additional subculturing is performed in the following manner.
Subculturing up to passage number 44 has been achieved using the methods of the present invention.
When such primary cells are subcultured, each round of subculturing is referred to as a passage.
Subculturing of hUCB cells decreased by half the time the cell confluence for 14 days.
The resulting strain was acclimated to minimal media by sequentially subculturing with a 1 % inoculum.
