Examples of 'supernatant fraction' in a sentence
Meaning of "supernatant fraction"
supernatant fraction: In scientific terms, supernatant fraction refers to the liquid or substance that remains above a solid residue after centrifugation or precipitation.
How to use "supernatant fraction" in a sentence
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supernatant fraction
The supernatant fraction is then filtered in a filter crucible.
All enzyme activity was confined to the supernatant fraction.
The supernatant fraction was then subjected to ammonium sulfate fractionation.
Bound radioactivity was determined by counting of supernatant fraction in scintillation fluid.
The supernatant fraction was recovered as anew soybean emulsion composition.
The percentage of each protein in the supernatant fraction was then quantified.
The supernatant fraction was used after centrifugation to remove the beads.
Most of the zinc remained in the supernatant fraction in both of these formulations.
The supernatant fraction and the fluid beneath the pellet were discarded.
The polysaccharide being neutral is recovered as the supernatant fraction in readiness for hydrolysis.
The high speed supernatant fraction was used as the receptor preparation.
The suspension is centrifuged in hematocrit capillary tubes to produce a pellet fraction and a supernatant fraction.
Radioactivity remaining in the supernatant fraction was determined with a scintillation counter.
HPLC analysis showed malonic acid only in the supernatant fraction.
Radioactivity remaining in the supernatant fraction was determined with a gamma or scintillation counter.
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Preferably, the crude lysate is centrifuged, and the supernatant fraction is recovered.
The supernatant fraction contained only the high molecular weight protein.
The antibiotic may be recovered from the supernatant fraction of the fermented broth.
The supernatant fraction was recovered and subjected to the purification protocol described below.
Specific and significant reactivity was observed in supernatant fraction from vpr transfected cells only.
Aliquots of the supernatant fraction and the chromatin fraction of each reaction were analysed.
The crosslinks are also reversed on the supernatant fraction from the IP.
This supernatant fraction can also be decanted under sterile conditions into a sterile container.
Repeated this step twice, saving each supernatant fraction in a separate tube.
The supernatant fraction was analyzed by Western blot.
For 5 min in the microcentrifuge, and the supernatant fraction was again aspirated.
The supernatant fraction was discarded and the pellet was resuspended in 5 ml of medium.
However, this precipitation is not complete since protein is found in the supernatant fraction.
In addition, the supernatant fraction may also be utilized as a vaccine component.
Representative clones are bioassayed for secretion of hIL-7 into the culture supernatant fraction.
The resultant supernatant fraction so obtained is designated the cell free extract ( cfe ).
Trypsin inhibitors, which are proteins, are found primarily in the supernatant fraction after dilution.
To the supernatant fraction 1L of a 2 percent solution of protamine sulfate was added.
The enzyme activity was determined in the refolded pellet fraction, and in the supernatant fraction.
The supernatant fraction retains releasing activity and most elutes in the early fraction from RP-HPLC.
For 5 min in the microcentrifuge, the supernatant fraction was drawn off.
The dried supernatant fraction was resuspended with 20 µl of sample solution.
The pH optimum of the PHB depolymerase in the supernatant fraction was about 8-9.
The supernatant fraction so obtained is designated the cell-free extract ( cfe ).
However, reductase activity appears to be even higher in the post-mitochondrial supernatant fraction ( Figure 2 ).
The clear supernatant fraction ( upper layer ) was transferred into a clean tube.
Nuclei were pelleted, and the cytoplasmic-containing supernatant fraction was precipitated with trichloroacetic acid ( TCA ).
The supernatant fraction was then assayed for total TGF-B1 content.
Two thiol - endopeptidases optimum pH 7.5 were purified from the supernatant fraction.
Aceton was added to the supernatant fraction to make final concentration of 66 %, whereby precipitation occurred.
The mixture was centrifuged at 4000xg for 15 minutes to remove free PEG in a supernatant fraction.
The soluble protein in the supernatant fraction was passed over a Ni - affinity column at 5 °C.
The supernatant fraction was subjected to SDS-PAGE ( reducing conditions ) with silver-staining.
Platelet-rich plasma was obtained from the supernatant fraction of blood after centrifugation at 1200 r . p.m.
The supernatant fraction was removed and stored frozen ( -30 ° C ) until assay for myeloperoxidase activity.
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Examples of using Supernatant
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Use the supernatant containing the antigens
A virus titration is performed on the harvested supernatant
The supernatant was carefully separated to a different tube
Examples of using Fraction
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That fraction does not make any sense
You do not have a fraction of her guts
A fraction of a day will be considered a full day
