Examples of 'synthetic gene' in a sentence
Meaning of "synthetic gene"
synthetic gene: A genetically engineered or artificially created gene that is not naturally occurring, often used in scientific research, biotechnology, or gene therapy to study specific traits, diseases, or biological processes
How to use "synthetic gene" in a sentence
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synthetic gene
The product of the synthetic gene is then produced recombinantly.
There are two major approaches to the construction of a synthetic gene.
A synthetic gene encoding this peptide is constructed in several steps.
Assembly of the synthetic gene.
Preparation of synthetic gene construct encoding dog urinary kallikrein.
The application further discloses a method of designing a synthetic gene.
The synthetic gene sequence was divided into four nucleotide fragments.
Use of an at least partially synthetic gene is preferable for several reasons.
The synthetic gene segments are then tested for improved expression.
The gene encoding the enzyme of interest may be a synthetic gene.
Such methodology of synthetic gene construction is well known in the art.
Construction of various mutants can be accomplished vÃa cassette mutagenesis of a synthetic gene.
Assemble synthetic gene segments and test for improved expression.
The result was a net improvement in codon usage frequency of the synthetic gene.
A hybrid plasmid containing the synthetic gene correctly inserted was thus isolated.
See also
The insulin gene may be mentioned at this point as an example of a synthetic gene.
The integrity of the ThXI synthetic gene was determined by sequencing of both strands.
The same is also true in comparing the modified gene to the synthetic gene.
First a synthetic gene is designed to embody each of the synthetic variable domains.
Four to six mutations were found in the synthetic gene from each clone.
The synthetic gene is assembled and inserted into the desired expression vector.
Khorana made his second scientific breakthrough with the construction of the first synthetic gene.
The synthetic gene on this plasmid does not contain the gac signal sequence.
The first strategy involved designing and constructing a totally synthetic gene that encoded alkaline phosphatase.
The synthetic gene is inserted in a suitable cloning vector and transfected into a host cell.
CDNA may be synthesized for example by reverse transcription or synthetic gene assembly.
The synthetic gene is inserted in a suitable cloning vector and recombinants are obtained and characterized.
These data demonstrate that the synthetic gene we designed encodes a functional NAGK enzyme.
The synthetic gene may even code for an engineered protein not known in nature.
Summary of the Invention The invention features a synthetic gene according to the appended claims.
The synthetic gene was constructed in vitro from eight synthetic oligonucleotides with partial overlaps.
TABLE XIV Use of a synthetic gene for the expression.
The synthetic gene is inserted into a suitable cloning vector and recombinants are obtained and characterized.
The child 's been injected with a synthetic gene mutation.
The invention also features synthetic gene fragments which encode a desired portion of the protein.
In a preferred embodiment of the invention, the nucleic acid is a synthetic gene.
Inserts representing parts of the synthetic gene are subsequently purified and sequenced using standard procedures.
In the current proposal, these ten peptides were assembled into a synthetic gene.
Assembly of the synthetic gene First synthetic block.
Synthetic gene transfer molecules can be designed to form multimolecular aggregates with plasmid DNA.
TABLE XV Use of a synthetic gene for the expression of.
A nucleotide sequence according to claim 3 wherein the nucleotide sequence is a synthetic gene.
An example of synthetic gene construction is set forth in the Examples.
A yeast cell genetically engineered to be able to express the synthetic gene of claim 35.
The synthetic gene control region is a specific DNA sequence.
Figure 4 depicts the nucleotide sequence of a synthetic gene encoding an ancrod-like polypeptide.
The synthetic gene was created using conventional DNA manipulation techniques.
In some embodiments, the synthetic gene may encode a modified protein.
The synthetic gene encoded histidine and asparagine, respectively, at these positions.
Figure 7 depicts yeast expression vectors containing the synthetic gene for I-SceI.
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