Examples of 'synthetic oligonucleotide' in a sentence
Meaning of "synthetic oligonucleotide"
synthetic oligonucleotide - a man-made piece of genetic material composed of short sequences of nucleotides, often used in laboratory research or diagnostic procedures.
How to use "synthetic oligonucleotide" in a sentence
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synthetic oligonucleotide
The complementary polynucleotide is preferably a synthetic oligonucleotide.
A matching synthetic oligonucleotide linker was inserted.
Additional embodiments include substitutions on the synthetic oligonucleotide.
Synthetic oligonucleotide duplexes used for the various constructions.
The oligonucleotide may be a naturally occurring oligonucleotide or a synthetic oligonucleotide.
A synthetic oligonucleotide can ligate to a fragment that has not been dephosphorylated.
The oligonucleotide can be a naturally occurring oligonucleotide or a synthetic oligonucleotide.
Both the transcriptase and the synthetic oligonucleotide are commercially available.
This synthetic oligonucleotide is radiolabelled and used to screen the gene library.
Remaining gaps were filled by sequencing with synthetic oligonucleotide primers.
Subsequently a synthetic oligonucleotide complementary to a portion contemplated to cause mutation is synthesized.
Similarly the binding can be altered by appropriate changes to the synthetic oligonucleotide.
Target was a synthetic oligonucleotide.
Synthetic oligonucleotide primers were prepared using commercially available automated oligonucleotide synthesizers.
These single strands are hybridized with a synthetic oligonucleotide mutated at the target site.
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The synthetic oligonucleotide is as described above in the description of the design process.
A single strand of the vector is then incubated in the presence of the synthetic oligonucleotide.
Synthetic oligonucleotide sequencing primers are prepared as necessary to achieve overlapping sequencing runs.
This is ensured by appropriate design of the terminal nucleotides of the synthetic oligonucleotide primers.
Iii target by a synthetic oligonucleotide a carcinogenic mutation and then correct it by another oligonucleotide.
A unique SalI site was inserted into the BamHI site using a synthetic oligonucleotide primer.
A significant drawback to the synthetic oligonucleotide approach is that it would require periodic administrations.
Modified base analogues provide another means of altering the characteristics of the synthetic oligonucleotide.
Synthetic oligonucleotide linkers and primers were prepared using commercially available automated oligonucleotide synthesizers.
This operation ensured that the library maintained the original complexity of the synthetic oligonucleotide.
Duplex synthetic oligonucleotide cassettes are then ligated into the gap between the restriction sites.
The method of inhibition of the C synthetic oligonucleotide is not known.
Synthetic oligonucleotide primers were designed on the basis of the coding sequence of each ORF.
The single stranded polynucleotide can be RNA or DNA and can also be a synthetic oligonucleotide.
Synthetic oligonucleotide methods of introducing point mutations can be preformed using heteroduplex DNA.
Alternatively, the antisense molecule is a synthetic oligonucleotide.
The synthetic oligonucleotide was prepared for use by reaction with ATP and polynucleotide kinase.
Usually, the extender probe is a synthetic oligonucleotide.
A synthetic oligonucleotide was prepared by using automatic DNA synthesizer with the principle of immobilized method.
In one embodiment, the vector is a synthetic oligonucleotide.
All synthetic oligonucleotide primers for PCR were by Biotech.
In one alternative, the oligonucleotide is a synthetic oligonucleotide.
Each synthetic oligonucleotide was then cut from the vector using Fok I restriction endonuclease.
Additionally, the test substance is a synthetic oligonucleotide.
All synthetic oligonucleotide primers for PCR were synthetized by MWG Biotech.
Screening using a synthetic oligonucleotide probe,.
The synthetic oligonucleotide of claim 3, further including a linker at a terminus.
Alternatively, in a preferred embodiment, the antisense molecule is a synthetic oligonucleotide.
Synthetic oligonucleotide templates are attached to a glass slide vía a 5 ′ amino modification.
The cDNA libraries were screened by hybridization with a synthetic oligonucleotide probe,.
Next, a synthetic oligonucleotide complementary to the site to be mutated is prepared.
The number of nucleotide residues in a synthetic oligonucleotide may in principle be any integer > 1.
Next, a synthetic oligonucleotide complementary to a portion intended to cause mutation is used.
In this method, the sample is contacted with a synthetic oligonucleotide of the invention.
It can be a synthetic oligonucleotide or constructed from a virus, plasmid, or the like.
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