Examples of 'target locus' in a sentence
Meaning of "target locus"
target locus - In various fields such as psychology or biology, this phrase refers to a specific point, area, or location that is the focus of a study, investigation, or analysis
How to use "target locus" in a sentence
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target locus
Cleaving a target locus in human cells.
Various constructs can be prepared for homologous recombination at a target locus.
Any such target locus can be from a rat.
Methods and compositions are provided for modifying a target locus in a cell.
The target locus can be either an extrachromosomal element or a stably integrated chromosomal element.
Long regions of homology to the target locus may be included in the vector.
It might as well begin before or after the start of the target locus.
Transgene integration at the target locus should have minimal or no effect on cell phenotype.
This can result in placing a desired new sequence at the endogenous target locus.
The gene mosaic is integrated into the target locus on a target chromosome.
The software is further capable of generating a consensus sequence for each target locus.
The target locus comprised the first five exons of the Cmah gene.
Further provided are methods and compositions are provided for modifying a target locus in a cell.
In one embodiment, the target locus is a genomic locus.
The list of highly expressed genes given above is also suited as target locus.
See also
Preferably, the target locus encodes an antibody heavy chain gene.
A typical mapping experiment employs linkage analysis of a target locus and genetic polymorphisms.
Direct visualisation of a target locus may use electron micrography, or fluorescence microscopy.
Identifying a recombinant host cell which exhibits a sequence alteration in the host target locus.
In another embodiment, the target locus is located in a vector in the cell.
Such methods can comprise identifying one cell having the targeted modification at a target locus.
In one embodiment, the target locus is in the genome of the cell.
These primers may contain sequences having homology with the sequences close to the target locus.
In another embodiment, the target locus of interest is located in a vector in the cell.
Such nuclease agents may promote homologous recombination between the targeting vector and the target locus.
In one embodiment, a target locus in a pluripotent rat cell is targeted for genetic modification.
Such a nuclease agent may promote homologous recombination between the targeting vector and the target locus.
As already indicated, the target locus may be substituted with the analogous human locus.
In one embodiment, the targeted genetic modification at the target locus is biallelic.
In some embodiments, the second target locus comprises a variable light chain antibody sequence.
In further embodiments, additional polynucleotides of interest can be integrated at the target locus.
Desirable criteria for a target locus for transgene placement can therefore be summarised as below,.
In specific embodiments, the genetic modifications result in a knockout of the desired target locus.
The immobilized primers, thus, include information about the target locus including particular allelic information.
In this manner, exogenous nucleic acid sequences can be inserted into a target locus.
In a preferred embodiment, the target locus of interest comprises DNA.
Meganucleases will thus cut cellular genomes only once, at the target locus.
In some embodiments, the first target locus comprises a VH or Va or VY sequence.
The 4-nucleotide insert created a restriction enzyme site upon insertion into the target locus.
Primers are designed flanking the FUT8 target locus as provided below.
Of the resulting progeny from crosses, approximately 25 % will be homozygous mutant / mutant at the target locus.
In some embodiments, the second target locus comprises a VI or VB or Vδ sequence.
Optionally, the 5 ′ and 3 ′ target sequences are within the genomic target locus.
A method for serial modification of a target locus in a cell, comprising,.
The target locus comprised exon 2 to the stop codon of the C5 ( Hc ) gene.
In other embodiments, the polynucleotide of interest integrated at the target locus encodes a nuclear protein.
Insertion of the insert polynucleotide at the target locus results in a “ modification of allele . ”.
Figure 17H to 17L depict nucleotide sequence analysis at the GMD target locus.
The method of claim 6 or 7, wherein the target locus encodes an antibody heavy chain.
In specific embodiments, the genomic locus of interest comprises a target locus of a “ rat nucleic acid ”.
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