Examples of 'template strand' in a sentence
Meaning of "template strand"
Template strand: In genetics, refers to the DNA strand that is used as a template for mRNA synthesis during transcription
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- The noncoding strand of a DNA molecule that is used as a template for RNA synthesis
How to use "template strand" in a sentence
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template strand
This strand is called the template strand.
From the template strand sequence.
Such primer usually complements the template strand precisely.
A template strand and companion primer is synthesized and is purified before use.
The other primer is complementary to a segment of the complementary template strand.
Methods for dissociation of the template strand include for example heat denaturation.
This allows for high efficiency of amplification through multiple initiation reactions from each template strand.
Thick lines represent RNA template strand that requires to be amplified.
The newly synthesized DNA strand is complementary to the template strand.
The template strand is then destroyed with UNG and AP endonuclease.
A primer must be sufficiently complementary to hybridize with a template strand for primer elongation to occur.
The template strand of the transcription bubble engages with the RNA polymerase II active site.
Related Images of what is a template strand.
Thus, the template strand and the new strand can be distinguished.
The double stranded DNA containing the template strand is no longer needed.
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In nature, the polymerization of ribonucleotides and deoxyribonucleotides is directed by a template strand.
Double stranded DNA acting as template consists of the template strand and the complementary strand.
Optionally the template strand can be removed by nuclease digestion, freeing the Xpandomer.
The bifurcated duplex is formed between a substrate strand and a template strand as shown in Fig.
Accordingly, the primer binds to a template strand and directs synthesis of the complementary strand.
Subgenomic mRNA 's are essentially smaller sections of the original transcribed template strand.
In some embodiments, a target nucleic acid serves as a template strand in a nucleic acid cleavage structure.
This dissociation constant will be altered, however, when pyrimidine is in the template strand.
This recognition occurs when the opposing template strand ( not the assembly template ) is methylated.
Preferably, a molar excess of the primer is admixed to the buffer containing the template strand.
Therefore, the terminal nucleotide is hybridized to the template strand when the oligonucleotide P * is annealed.
Preferably, a molar excess of the oligonucleotides over the DNA template strand.
Strand 11 is a template strand that can be transcribed into a NtHMA RNAi polynucleotide 13.
On the other hand, placing a pyrimidine in the template strand inverts the base pair.
The subgenomic RNA, which codes the structural proteins, is transcribed from the negative template strand.
One femtomole of the template strand 40 bases in length is added to the mixture.
By treating the anchored double-stranded PCR product with alkali the non-anchored template strand is removed.
In this case, the template strand has a covalently attached sterol moiety e . g.
Unlike RNA, DNA polymerases can not synthesize DNA from a template strand.
Within a duplex the template strand is, by convention, depicted and described as the " bottom " strand.
Figure 10 shows a method for preparing a template strand.
In the non-coding template strand as " AUG.
Treatment with the glycosylase releases uracil, producing lethal apyrimidinic ( AP ) sites in the template strand.
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