Examples of 'terminal transferase' in a sentence
Meaning of "terminal transferase"
Terminal transferase: A template-independent DNA polymerase enzyme that adds deoxynucleotides to the 3' hydroxyl terminus of DNA. Commonly used in molecular biology techniques like DNA sequencing and PCR
How to use "terminal transferase" in a sentence
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terminal transferase
Terminal transferase has applications in molecular biology.
A preferred method of labeling is tailing by terminal transferase labeling.
Terminal transferase catalyzes the addition of nucleotides to the 3 ′ terminus of a nucleic acid.
It is difficult to control how many bases are appended by terminal transferase.
This method takes advantage of the terminal transferase activity of reverse transcriptases.
These may be provided by attaching a polymeric sequence by employing the enzyme terminal transferase.
In some embodiments, the non-template terminal transferase is a reverse transcriptase or a polymerase.
Or the reagents could include nucleotides for a polymerase or terminal transferase reaction.
The terminal transferase can include a dideoxynucleotide triphosphate, methylated nucleotide triphosphate, and the like.
Soybean genomic DNA fragmented by sonication and elongated using terminal transferase.
In humans, terminal transferase is encoded by the DNTT gene.
The TA method takes advantage of Taq polymerase terminal transferase activity.
The appropriate enzyme - terminal transferase - will add nucleotides to the free ends of DNA molecules.
The fragmented DNA amplicons were subsequently labeled with biotin by Terminal Transferase.
A kit containing a terminal transferase and biotinated deoxyuridine 5 ' - triphosphate can be purchased from Boehringer Mannheim.
See also
In particular embodiments, a reverse transcriptase can comprise a non-template terminal transferase activity.
This 3 ' OH end was extended with terminal transferase by adding a poly-A tail.
In an embodiment of the methods, adding a polynucleotide tail comprises the use of a terminal transferase.
DdG denotes a dideoxy G that was added using terminal transferase ( post oligonucleotide synthesis ).
One solution is to convert flush-ended molecules to sticky-ended molecules by enzyme terminal transferase.
A poly-dC tail was added to the cDNA using terminal transferase ( TdT ; Life Technologies ).
Reagents for the terminal transferase assay were purchased from Boehringer-Mannheim Biochemicals, Indianapolis, IN.
The first strand cDNA is isolated and tailed with oligo ( dG ) using terminal transferase.
Terminal Transferase Reaction ( TdT ).
This product is then G-tailed using terminal transferase.
Similarly, the DNA sequence may be tailed with cytosine residues utilising terminal transferase.
The FPET cDNA is then tailed with Terminal Transferase ( TdT ) and dATP.
The method of claim 10, wherein adding a polynucleotide tail comprises the use of a terminal transferase.
End labeling can be performed using terminal transferase ( TdT ).
Addition of a poly ( dX ) oligonucleotide to the 3 ′ ends of the DNA fragments using a terminal transferase.
The reaction was stopped by heat inactivation of the terminal transferase at 70°C for 10 min.
The 3 ' terminal dideoxynucleotide was added by terminal transferase.
The NS5b region has both RNA-dependent RNA polymerase and terminal transferase activity.
Stochastic synthesis using the enzyme Terminal Transferase ( TdT ).
Then, a 3 ' poly-dG tail can be added with terminal transferase.
For example, the end link primer may be a homopolymer added by, e.g., terminal transferase.
Labeling of Probes with 32P-cordycepin and terminal transferase.
Revelation by hybridization of the PCR products obtained after denaturation / renaturation ( 1 ) or 2 terminal transferase steps ( 2 and 3 ).
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Terminal transferase has applications in molecular biology
Then sugars are added one at a time by glycosyl transferase
The glucuronosyl transferase is apparently induced by rifampin
