Examples of 'thermocycling' in a sentence
Meaning of "thermocycling"
thermocycling: the process of repeatedly changing the temperature of a substance, commonly used in scientific experiments such as polymerase chain reaction (PCR)
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- The use of a thermocycler to amplify DNA segments
How to use "thermocycling" in a sentence
Basic
Advanced
thermocycling
System for thermocycling of fluids in cartridges comprising.
The droplets can remain intact during thermocycling.
The standard TaqMan thermocycling protocol was used.
Thermocycling of a block comprising multiple sample.
Testing method for thermocycling amplification.
Thermocycling of a block comprising multiple samples.
The vessels are ready for thermocycling.
Thermocycling is preferentially done in a computer controlled manner.
In an embodiment there are provided two or more thermocycling chambers.
Forty cycle thermocycling sequences were conducted.
Nucleic acids may be amplified by a variety of thermocycling and isothermal techniques.
Multiple thermocycling runs are required to determine an efficient balance of reagents.
The temperature variation with time is summarized by the thermocycling protocol.
The means for thermocycling can be chosen arbitrarily.
The complex is resolved in a preheating step prior to the thermocycling process itself.
See also
This rapid thermocycling technique uses thin glass cuvettes as reaction vessels.
There are a number of techniques for amplifying nucleic acid which involve thermocycling.
A method for the simultaneous thermocycling of multiple samples comprising the steps.
Thermocycling releases the primers so they can function in additional cleavage reactions.
A nucleic acid library can be amplified by a thermocycling method or by an isothermal amplification method.
Thermocycling during the reaction is performed as described in the temperature control section supra.
In a preferred embodiment the thermocycling chambers are only used for the thermocycling step.
The mixture can be pipetted or collected into a standard reaction tube for thermocycling.
At the end of the thermocycling an extended incubation was added for the hybridization.
The longevity of the bond was generally studied by thermocycling the materials in water.
Using thermocycling the ligated oligonucleotides then become a target for further oligonucleotides.
The trigger reaction may be run under conditions that allow for thermocycling.
The methods relate to the use of modified thermocycling procedures for achieving improved amplification results.
It provides a flexible surface coating which eliminates oil permeability due to thermocycling in use.
During nucleic acid amplification and detection thermocycling of the amplification and detection chambers takes place.
The reaction conditions which were most effective at preserving catalytic activity during thermocycling were identified.
The thermocycling is repeated until the desired amount of PCR product is produced.
The skilled person will recognise that variations of this thermocycling reaction may be made.
PCR thermocycling reactions were performed using the following procedure and conditions.
PCR reactions were subjected to thermocycling as described above.
Thus DNA can be amplified at a single temperature without the use of thermocycling.
The same PCR thermocycling conditions as described for the masterplate screening were utilized.
The size and distribution of complexes during thermocycling was monitored by GPC.
The same PCR thermocycling conditions as described for the masterplate screening were utilised.
The individual PCR reactions are performed simultaneously in individual tubes or in thermocycling reaction plates.
Thermocycling can induce first strand cDNA synthesis from the RNA transcript target.
Fluorescence recording is not required during PCR thermocycling.
Thermocycling was performed on a Quanta Bioscience slide block thermocycler.
NEAR amplification has similarities to PCR thermocycling.
PCR and thermocycling were performed using standard recombinant DNA methodology.
The Linear Beacon was added to the PCR cocktail before thermocycling.
Chip loading and thermocycling conditions were according to the Life Technologies instructions.
Group 2 was not exposed to thermocycling procedure.
Thermocycling and Environmental testing.
No incubation time or thermocycling required3.
