Examples of 'thermolysin' in a sentence
Meaning of "thermolysin"
thermolysin (noun) - A protease enzyme that breaks down proteins by hydrolyzing peptide bonds, often used in research and biotechnology
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- A thermostable neutral metalloproteinase enzyme produced by the gram-positive bacterium Bacillus thermoproteolyticus.
How to use "thermolysin" in a sentence
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thermolysin
The numbering is based on the thermolysin sequence.
Thermolysin can be obtained as a lyophilisate from commercial suppliers.
Unreconstituted lyophilized CLECs of thermolysin appear to be viable indefinitely.
Thermolysin is an aggressive protease which in solution undergoes autoproteolytic attack.
Stabilization of thermolysin in aqueous solution.
Several modelling experiments were conducted using different thermolysin concentrations.
The thermolysin peak was quantified relative to the other peaks obtained.
The conformation of thermolysin.
Thermolysin CLECs retained maximum activity after five days incubation at elevated temperature.
The gel time then also depends on the thermolysin concentration used.
The first main step is the formation of a clear solution containing thermolysin.
The remainder comprised more heavily degraded thermolysin fragments and other impurities.
The kit may also contain in a separate sealed compartment a lyophilized preparation of a thermolysin.
Soluble thermolysin and thermolysin CLEcs exhibited markedly different stability in the presence of organic solvents.
The same effect is observed for frozen thermolysin solutions according to the invention after thawing.
See also
In both cases it is possible to use Thermolysin.
Subtilisin Carlsberg and thermolysin were used as references for comparison.
The protein concentrations detected therefore correspond to mixtures of intact thermolysin and degradation fragments thereof.
Thermolysin is used in the manufacture of the artificial sweetener aspartame Isowa et.
Due to the transition, solubility of thermolysin decreases.
The thermolysin CLECs retained greater than 95 % maximum activity following incubation with all organic solvents assessed.
The protease used is the thermolysin used in example 1.
The method of claim 5 wherein the endoprotease is thermolysin.
For example, the term thermolysin includes crude thermolysin.
A metalloproteases protease may for example be a thermolysin from e . g.
The neutral protease is preferably thermolysin or dispase, and most preferably is dispase.
Inhibitors have also been disclosed for the related protease, thermolysin.
Therefore, no coefficient was applied to the thermolysin concentration in the equations.
In order to limit autoproteolytic attack, low temperatures are applied to solutions containing thermolysin.
Fibronectin was digested with thermolysin and then passed over a gelatin-SEPHAROSE column.
The blend may, by way of example comprise a further neutral protease such as thermolysin.
Thus, ACE is a member of the thermolysin branch of the zinc metalloproteases.
Thus, the chromatogram after diafiltration indicated substantial separation and purification of undegraded thermolysin.
Certain proteases such as dispase II, trypsin, and thermolysin may be of use in decellularization.
The stability of Tn3 to proteolytic degradation was tested by incubation with thermolysin.
Thermolysin is an extracellular, metalloendopeptidase secreted by the gram-positive thermophilic bacterium Bacillus thermoproteolyticus.
Suitable metalloproteases can include collagenases, hemorrhagic toxins from snake venoms and thermolysin from bacteria.
In contrast, thermolysin CLEC concentrations were limited only by the volume occupied by the crystals.
A process as in Claim 1 wherein the enzyme is thermolysin.
The Sulfolobus tokodaii scaffold exhibited thermolysin resistance over the entire 16 hour time course.
CLEC enzyme is defined as reconstituted lyophilized cross-linked thermolysin crystals.
The washed cross-linked thermolysin crystals constitute the thermolysin CLEC used below as a catalyst.
Most preferred protease is a protease derived from a prokaryotic source, such as pronase, subtilisin A or thermolysin.
NaJ . Thermolysin is an aggressive protease which in solution undergoes autoproteolytic attack.
The second method included proteolysis of the activin A preparation with Lysine C, chymotrypsin, pepsin and thermolysin.
Thermolysin Bacillus thermoproteolyticus ; species variants include Micrococcus caseolyticus and Aspergillus oryzae.
The reaction was initiated with 15 mg thermolysin added as a solid.
The purity of thermolysin in the dry THERMOASE powder was about 20 %.
The enzyme crystal according to claim 1, wherein said enzyme is thermolysin.
The protease used is the thermolysin used in examples 1 and 2.
