Examples of 'top agar' in a sentence
Meaning of "top agar"
top agar - This phrase does not make sense in English. 'Top' could refer to something at the highest point, while 'agar' can refer to a gelatinous substance used in laboratories
How to use "top agar" in a sentence
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top agar
Fresh top agar was added once a week.
Swirl the plate quickly so the top agar covers the entire plate.
Antibodies were administered at the time of suspension in both bottom and top agar solutions.
Allow the top agar to solidify on the bench.
Different dilutions of the cell free phage isolates are then spotted on the top agar.
The buffer was recovered and the top agar was also scraped into a centrifuge tube.
To these tubes were added 3 ml of top agar.
If the top agar tends to harden prematurely, the bottom agar plates can be prewarmed.
Ensure a smooth, even distribution before the top agar hardens.
Add top agar to each tube to a final volume of 45 ml.
Colonies may be picked through the top agar even 5 days later.
The top agar is allowed to harden for 10 minutes at room temperature.
Antibodies or a control solution ( PBS ) was included in bottom and top agar solutions.
The top agar was allowed to solidify and the plates were incubated overnight at 37°C.
Before it was used the top agar was melted and cooled to 48°C.
See also
Top agar technique,.
The transfected cells were overlaid with top agar and cultured at 32 °C for 14 days.
Once the top agar has solidified, the plates are incubated in an inverted position at 30°C.
Wild type cells of Synechocystis are mixed with equal volumes of 2x top agar and 2x BG-11.
Then, 6 ml of top agar the SC medium of Sherman et al.
After plaque formation, the plates were incubated at 4°C for ∼ 1 hour to harden the top agar.
Allow top agar to solidify completely ( approximately 30 minutes ), then invert plate.
After the top agar hardened, the plates were incubated at 37 °C overnight.
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Examples of using Top
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You have been on my top two thoughts as well
Top of his class in the school of infantry
We took their six top department heads
Examples of using Agar
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Agar is odourless or has a slight characteristic odour
Decomposing ability of agar and alginate was negative
Agar consists of a mixture of agarose and agaropectin
