Examples of 'trabid' in a sentence
Meaning of "trabid"
trabid (noun): A type of small dog breed known for its playful and energetic nature. Trabids are often kept as pets and are adored for their friendly demeanor and loyalty
How to use "trabid" in a sentence
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trabid
Trabid has been identified as a new deubiquitylase enzyme.
This led to the identification of a protein called Trabid.
Trabid is preferably prepared by recombinant means.
Preferably the amount of Trabid in each treatment is the same.
Trabid protein does not have any biological function disclosed in the prior art.
A method for identifying a modulator of Trabid said method comprising.
Trabid inhibitor means inhibitor of Trabid function.
Contacting said first and second portions with Trabid.
Use of Trabid in the precipitation of a polypeptide comprising ubiquitin.
Preferably assays of the invention are assays for identification of inhibitors of Trabid.
Thus Trabid activity is assayed according to the present invention.
This finds particular application in the assessment of Trabid deubiquitylase activity.
The function of Trabid is disclosed herein for the first time.
We disclose for the first time the biological activity of Trabid as a deubiquitylase.
Trabid means the Trabid polypeptide or nucleic acid encoding same.
See also
We have generated a deletion of Drosophila Trabid by homologous recombination.
Fragments of Trabid may also find application in the present invention.
The protein identified is Trabid.
Providing a Trabid substrate comprising a detectable moiety coupled to a tag moiety by ubiquitin.
Epistasis experiments suggest a function for Trabid in nuclear Wnt signalling.
Therefore individual Trabid NZFs appear equivalent or even interchangeable for some applications.
Therefore, we focused our further analysis on Trabid.
Suitably the Trabid inhibitor may be an inhibitor of deubiquitinase activity such as ubiquitin aldehyde.
First the DUB activity of recombinant Trabid in vitro should be taken into account.
Trabid includes fragments of Trabid.
In this example the two Trabid inhibitors used are siRNAs to Trabid.
Trabid is a nucleo-cytoplasmic protein with deubiquitylating activity.
We further show that Trabid promotes Wnt signalling in mammalian cells.
Figure 4 shows evidence demonstrating the deubiquitylase activity of Trabid.
The candidate mutant Trabid polypeptide may be a library of multiple candidate mutant Trabid polypeptides.
Moreover, assays for inhibitors of Trabid activity are demonstrated.
Preferably Trabid polypeptide comprises the Trabid deubiquitylase active domain.
Figure 3 shows a range of biochemical results establishing Trabid function.
Our results implicate Trabid as a molecular target for inhibitory drugs in colorectal cancer cells.
Preferably said modulation is by steric hindrance brought about by Trabid binding e . g.
Preferably Trabid refers to the polypeptide.
The same applies to nucleotide-sequences for Trabid referred to herein.
Preferably Trabid is recombinant or purified Trabid.
Next, the plates are incubated to allow any Trabid action to take place.
Preferably Trabid modulation is Trabid inhibition.
Thus the invention relates to the downregulation of c-MYC by reduction of Trabid acivity.
Preferably Trabid comprises full length human Trabid polypeptide.
In this example, methods of identifying inhibitors of the deubiquitylase activity of Trabid are demonstrated.
Furthermore, Trabid has been biochemically characterised.
Identity is preferably judged along the length of the Trabid ( or fragment thereof ) of interest.
This argues that Trabid acts to control coactivator recruitment to the TCF-B-catenin complex.
An in vitro method of reducing TCF transcription, said method comprising reducing Trabid activity.
A method according to claim 1, wherein Trabid activity is reduced using siRNA to Trabid.
The second tag is advantageously used in detection of release following Trabid cleavage ( if any ).
In the above examples, the function of Trabid in TCF-mediated transcription was investigated.
