Examples of 'tracrrna' in a sentence
Meaning of "tracrrna"
tracrrna (adjective): A misspelling or typo of the term 'tracrRNA,' which stands for 'trans-activating CRISPR RNA.' TracrRNA is a small RNA molecule that forms a complex with CRISPR RNA (crRNA) to guide the CRISPR-associated protein Cas9 to specific target sequences in the genome for gene editing
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- Initialism of w:Trans-activating crRNA.
How to use "tracrrna" in a sentence
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tracrrna
A tracrRNA can refer to a minimum tracrRNA sequence.
Alignment of the conserved repeat sequences and tracrRNA.
The tracrRNA can remain hybridized to the crRNA.
Sequences for the mature crRNA and tracrRNA are shown.
A tracrRNA can be modified by endogenous RNaseIII.
The crRNA and the corresponding tracrRNA hybridize to form a gRNA.
Forms of tracrRNA can include primary transcripts or processed forms.
Northern blot analysis of crRNA and tracrRNA expression in mammalian cells.
A subject gRNA can comprise any corresponding crRNA and tracrRNA pair.
Hybridization of the tracrRNA to the palindromic repeat sequence triggers processing.
Stable hairpin secondary structure between tracrRNA and direct repeat.
A part of the minimum tracrRNA sequence can hybridize to the minimum CRISPR repeat sequence.
A gRNA can comprise a nucleic acid sequence encoding a crRNA and a tracrRNA.
Very low amounts of long tracrRNA are detected on the Northern blot.
The guide RNA may be a dualRNA comprising a crRNA and a tracrRNA.
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The CRISPR RNA and tracrRNA transcripts were assembled in to duplex.
A first hairpin can comprise a duplex between the minimum CRISPR repeat and the minimum tracrRNA sequence.
The CRISPR RNA and the tracrRNA are introduced separately.
Type II systems also encode a tracrRNA.
In a preferred embodiment the tracrRNA sequence is 30 or more nucleotides in length.
As such, each crRNA can be said to have a corresponding tracrRNA.
Figure 35 depicts a sequence alignment of tracrRNA orthologues demonstrating the diversity of tracrRNA sequences.
Accordingly, a gRNA comprises a sequence that hybridizes to a target sequence, and a tracrRNA.
The crRNA and the corresponding tracrRNA ( as a corresponding pair ) hybridize to form a gRNA.
As described herein, in embodiments of the present invention, the tracrRNA is not required.
The tracrRNA of the complex can hybridize to a crRNA repeat in the pre-crRNA array.
In some embodiments, the tracRNA sequence is homologous to a tracrRNA over 6 contiguous nucleotides.
In one embodiment, the gRNA comprises a nucleic acid sequence encoding a crRNA and a tracrRNA.
The tracrRNA is required for crRNA maturation from a primary transcript encoding multiple pre-crRNAs.
By contrast Cas9 requires both crRNA and a transactivating crRNA tracrRNA.
The cognate tracrRNA can be identified following the method described in Example 6.
In some embodiments, the continuous RNA molecule comprises a tracrRNA sequence.
In one embodiment, the crRNA and the tracrRNA are expressed as separate RNA transcripts.
In some aspects, the continuous RNA molecule comprises a tracrRNA sequence.
TracrRNA is partially complementary to and base pairs with a pre-crRNA forming an RNA duplex.
In some embodiments, the linker sequence links the CRISPR repeat and the tracrRNA sequence.
More specifically, tracrRNA and CRISPR RNA transcripts were obtained as described in Example 1.
In some aspects, the linker sequence links the CRISPR repeat and the tracrRNA sequence.
Advantageously, the tracrRNA sequence is 30 or more nucleotides in length.
In some of the above methods, the CRISPR RNA and the tracrRNA are introduced separately.
For example, the guide RNA of type II CRISPR / Cas systems can comprise crRNA and tracrRNA.
In other methods, the CRISPR RNA and the tracrRNA are introduced separately.
A 3 ′ tracrRNA sequence can comprise two duplexed regions.
Figure 17 shows the co-fold structure of the tracrRNA and direct repeat.
The putative tracrRNA element for this CjCas9 is,.
In some embodiments, the first polynucleotide comprises at least 50 % identity to a tracrRNA over 6 contiguous nucleotides.
Wherein the tracrRNA sequence is 50 or more nucleotides in length.
The vector already contains the partial direct repeat ( gray ) and partial tracrRNA ( red ) sequences.
A tracrRNA can refer to a mid-tracrRNA.
Figure 22 demonstrates that tracrRNA is required for target DNA recognition.
