Examples of 'transfectants' in a sentence
Meaning of "transfectants"
transfectants (noun): In biology, transfectants are cells that have undergone the process of transfection, where genetic material has been introduced into the cell
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- plural of transfectant
How to use "transfectants" in a sentence
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transfectants
Stable transfectants were selected according to hygromycin resistance.
This allows the analysis of single cell transfectants.
Transfectants were selected in growth medium containing methotrexate.
The obtained cells were used as transfectants for immunogens.
Transfectants were transiently transfected to assess IgG productivity.
Inward barium currents were detected in two of these nine transfectants.
Transfectants were cloned by the limiting dilution method.
This technique is known to create stable transfectants at a high frequency.
Positive transfectants that showed high yield were banked.
The effect of antibody treatment on the transfectants was compared to medium alone.
Transfectants were selected in medium lacking hypoxanthine and thymidine.
Experiments show that CDase transfectants are indeed expressing active enzyme.
The transfectants were tested for antibody secretion and specificity.
A selectable marker gene to facilitate recognition of transfectants may also be incorporated.
Consequently the transfectants isolated were poor antibody producers.
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The process to obtain these is similar to that used to derive primary transfectants.
Mock transfectants were generated as negative controls.
The product secreted by two individual transfectants were analyzed for chain composition.
Stable transfectants were obtained in the same manner as above.
Cell membrane preparations were collected from the stable transfectants described above.
Mock transfectants were used as negative controls.
A variation on this format is to challenge transfectants with virus and select for survivors.
Stable transfectants were isolated by positive selection in medium containing geneticin.
These clones produced superoxide constitutively and spontaneously as observed in the transient transfectants.
Transfectants expressing high level of receptors were cloned by limiting dilution.
They are useful when stable transfectants are desired as a continuous source of antibody.
Transfectants were prepared in duplicate.
This was tried with two transfectants and was successful with one of them.
Transfectants were enriched using the same condition as in the first screening.
Various methods produce stable transfectants which express the CARs of the invention.
The transfectants are selected in growth medium containing methotrexate and neomycin.
The wells were washed and supernatant from the transfectants was added to the plate.
Eukaryotic transfectants were then produced.
Splenocytes from immunized mice were cultured for five days with irradiated transfectants in vitro.
The transfectants were analyzed or frozen.
The three different preparations were added to transfectants expressing hirudin tethered to the cell surface.
The transfectants were selected in growth medium containing methotrexate and neomycin.
For each transfected cell line selection of stable transfectants was carried out with geneticin.
Transfectants with stable expression were selected using the antibiotic blasticidin.
Supernatants from the stable transfectants can reduce this increase in tubule formation.
Transfectants are recovered in selective medium containing xanthine and mycophenolic acid.
Antibody production and activity assays for the stable transfectants were performed as described below.
The majority of transfectants produce less than maximal levels of the desired protein.
A similar result was obtained with a second set of independently isolated rabbit growth hormone receptor transfectants.
Supernatants from stable transfectants were assayed for the presence of the chimeric antibody.
DR aB heterodimer was detected in the cell lysates and supernatants of DR aB transfectants.
This procedure selects for transfectants with an elevated number of copies of integrated plasmid.
These expression vectors also contain the dihydrofolate reductase gene for selecting stable CHO transfectants.
Transfectants detected in this manner appear as dark foci against a relatively light background.
Transcription of the construct in the transfectants was confirmed by Northern blotting analysis.
