Examples of 'transfection reagent' in a sentence
Meaning of "transfection reagent"
Transfection reagent: A substance used in biological research to introduce nucleic acids into cells. It helps facilitate the transfer of genetic material into a cell, commonly used in techniques such as transfection and gene editing
How to use "transfection reagent" in a sentence
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transfection reagent
With the use of transfection reagent.
No transfection reagent was added to control wells.
Without the use of transfection reagent.
Transfection reagent and then cells are added to each well.
No fluorescence was detectable in transfection reagent only treated wells.
No single transfection reagent or method works for all cell types.
Desired amount of siRNA was added on the diluted solution of transfection reagent.
All experiments using a liposomic transfection reagent showed positive results.
As transfection reagent.
Plasmid DNA is transfected into the cells using a suitable transfection reagent.
Protocols for each transfection reagent are available from the manufacturer.
RNAiMAX was a commercial transfection reagent.
Each type of transfection reagent represents a separate example of the present description.
In one embodiment, the therapeutic comprises a transfection reagent.
Each type of transfection reagent represents a separate embodiment of the present invention.
See also
The transfections were performed with the lipid transfection reagent lipofectAMINE ( GibcoBRL ).
The transfection reagent also mediates the binding and internalization of polynucleotides into cells.
In another embodiment, the transfection reagent is calcium phosphate.
In certain examples, the purified RNA preparation comprises a transfection reagent.
The STEMFECT transfection reagent was used as previously described.
For intravitreal injection, the siRNA was delivered in the absence of transfection reagent and rAdenovirus.
The transfection reagent used is DOTAP.
In another embodiment, the transfection reagent forms a liposome.
In certain embodiments, the purified RNA preparation comprises a transfection reagent.
DNA and a PEI transfection reagent were thawed in a sterile biosafety hood.
Medium was replaced daily prior to transfection of reprogramming mRNAs using RNAiMAX transfection reagent ( Invitrogen ).
In another example, the transfection reagent is calcium phosphate.
Oligonucleotides were delivered into cells in form of complexes with the Lipofectin ™ transfection reagent ( Invitrogen ).
In another embodiment, the transfection reagent is a lipid or lipidoid.
C6 cells in serum containing medium were transfected with the DNA using the transfection reagent Lipofectamine.
In another example, the transfection reagent forms a liposome.
PEAK cells were transfected with the modified pEAK8 vectors using Mirus transfection reagent.
In a further embodiment, the transfection reagent comprises a cation.
By “ reagent-based transfection ” is meant transfection using a transfection reagent.
In some embodiments, the transfection reagent comprises a nanoparticle or nanotube.
Polyethylenimine ( PEI ) was used as transfection reagent.
In some examples, the transfection reagent comprises a nanoparticle or nanotube.
PNAs were applied to 1 ml culture medium, without any transfection reagent.
In another embodiment, the transfection reagent is a protein-based transfection reagent.
Recombinant baculovirus ( BV ) was generated through co-transfection using the BaculoGold Transfection reagent ( PharMingen ).
In another embodiment, the transfection reagent is a polyethyleneimine based transfection reagent.
The vector solution was mixed with Lipofectamine 2000 transfection reagent in equal amounts.
In another example, the transfection reagent is a polyethyleneimine based transfection reagent.
The plasmid was transfected into the cells using transfection reagent FuGENE6 ( Promega ).
In one embodiment, the transfection reagent is incubated with a complexation medium.
Protocol ( c ), Transfection of Vero cells using DOTAP transfection reagent.
In another example, the transfection reagent is a protein-based transfection reagent.
The transient transfections were carried out using the FuGene 6 transfection reagent ( Roche, Basle, Switzerland ).
In another example, the transfection reagent is a lipid-based transfection reagent.
A preferred PEI is JetPEI, a commercially available linear polyethylenimine cationic polymer transfection reagent ( Qbiogene, Inc., CA ).
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Examples of using Transfection
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Magnetic nanoparticle transfection is the technical term
The transfection efficiency is tested at variable times
Several methods of cell transfection exist at present
Examples of using Reagent
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The reagent introduction location and conditions
It is a strong fluorination reagent and it is dangerously reactive
Reagent consumption and dosing activity counters
