Examples of 'transient transfection' in a sentence

Meaning of "transient transfection"

In molecular biology, transient transfection refers to the introduction of foreign genetic material, such as DNA or RNA, into cells for a short period of time. This method is often used to analyze gene function or protein expression in cell-based experiments

How to use "transient transfection" in a sentence

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transient transfection
Agrobacterium for transient transfection of whole plants.
The transfection may be a stable or a transient transfection.
Experimentally transient transfection can be performed essentially as described here.
Suitable for stable and transient transfection.
Transient transfection analysis.
The first method was a method of transient transfection.
Cell viability and transient transfection efficiency data were obtained.
Plasmids are particularly useful in producing transient transfection.
The test is performed by transient transfection into the clones to be tested.
High titer retrovirus stocks following transient transfection.
Transient transfection studies.
The protein was expressed by transient transfection as described herein.
It may be desirable to enrich the target cells prior to transient transfection.
Transient transfection has numerous advantages over the packaging cell method.
The siRNAs can also be introduced into cells vía transient transfection.

See also

Transfection methods for stable or transient transfection are commonly known in the art.
Recombinant proteins were then expressed in cultured mammalian cells by transient transfection.
The results obtained from the transient transfection assays could partially be confirmed.
Recombinant proteins are then expressed in cultured mammalian cells by transient transfection.
Transient transfection can also be used to measure vector production when vectors are being developed.
These mutants were tested for protein expression levels in transient transfection assays.
Transgene expression after transient transfection was increased when the expression plasmid contained oriP.
All five human mutants were effectively expressed and secreted after transient transfection.
Activities of these promoters were evaluated in transient transfection assays using luciferase as the reporter.
Initial transient transfection experiments were carried out to test protein expression from the pDRHK vector.
A useful alternative to stable expression is transient expression following transient transfection of an expression plasmid.
Transient transfection of the COS cells was performed as follows.
The host cells may be suitable for transient transfection and for stable transfection.
Comparison of expression efficiencies of various EPO expression vectors by transient transfection.
This procedure is highly suited for transient transfection used for various molecular biology studies.
The four different expression vectors described above are used for transient transfection of cells.
Promoter activity is tested by transient transfection of the obtained constructs into PER.
The transient transfection methods were adapted from the standard Qiagen Polyfect protocol.
The miRNA inhibitors may be contacted with the cells using transient transfection techniques.
Transient Transfection InVivo.
Functional expression of IRAP assessed in transient transfection experiments.
Nevertheless, transient transfection is also contemplated.
Knock down of endogenous human PVRIG was carried out by transient transfection of siRNA.
Preferably, the transient transfection method may be used.
Figure 7 shows cellular localization of pCMVLacZ after transient transfection.
The present invention is used for transient transfection of plants with a DNA sequence of interest.
Figure 8 demonstrates high levels of FcMBL expression from transient transfection.
Promoter activity is then analysed by transient transfection of the obtained luciferase constructs into PER.
Transient transfection is usually efficient, and expression can be observed almost immediately.
The different molecules were tested in transient transfection in the lines PER.
Utilizing a transient transfection procedure, rAAV devoid of adenovirus has been generated.
The expression of HA proteins was carried out by transient transfection with the expression vector.
We performed a transient transfection to express both the wild type and mutated mlh1 gene.
Method 2 is suitable for a transient transfection.
For example, transient transfection methods can be used.

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