Examples of 'transposase' in a sentence
Meaning of "transposase"
transposase (noun) - an enzyme that catalyzes the movement of a transposable element within a genome, allowing genes to be rearranged or activated/deactivated
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- An enzyme required for the transposition of transposons
How to use "transposase" in a sentence
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transposase
Addition of transposase assembles a transposome of the surface.
The transpositions are catalyzed by several transposase enzymes.
This enzyme can be a transposase or a sitespecific recombinase.
Intergrating enzymes can be any transposase.
Development of MuA transposase activity assay.
The transposase gene means a gene encoding a transposase.
Both operons are flanked by residual transposase fragments.
The addition of transposase is to be done subsequent to isolating bound chromatin.
Several mechanisms are disclosed to provide a source of the transposase.
The transposase ensures all the stages of displacement.
In some embodiments the recombinase is a transposase.
To prevent transposase production.
This usually requires a set of enzymes including transposase to be translated.
Any transposase system can be used in accordance with the embodiments.
Each of the domains separately is unable to provide for transposase function.
See also
Expression of the transposase and subsequent integration occurs at the desired target.
The selection of the gene coding for a transposase is not critical.
A transposase of the invention also includes integrases from retrotransposons and retroviruses.
The transposome can be attached vÃa the transposase or vÃa the transposon nucleic acid.
Transposase was added to assemble a transposome that did not contain any exposed dsDNA.
The present method avoids the need to produce an endogenous transposase molecule.
The transposase gene may be placed under the control of an inducible promoter.
It is understood that the chimeric integrating enzyme can be any chimeric transposase.
In some embodiments a transposase may be modified to lack the ability to form a dimer.
Such vectors may be introduced into cell lines having a source of transposase.
A transposase of the provided methods also includes integrases from retrotransposons and retroviruses.
This test enables the absence of the gene encoding the transposase to be verified.
In each case the transposase gene may be placed immediately downstream of a regulable promoter.
Both types of transposition are facilitated by the MuA transposase and accessory MuB protein.
The source of the transposase can include a nucleic acid encoding the transposase.
The first sequence is necessary for loading the oligonucleotide onto the transposase.
Portion is now completely stable because no transposase gene has been introduced into the plant.
Transposons are bracketed by terminal inverted repeats that contain binding sites for the transposase.
The said nucleic acid regions protected from transposase insertion may be identified by computational methods.
The nucleic acid construct further can include a nucleic acid sequence encoding a transposase.
The transposase expression vector and the corresponding transposon vector are cotransfected into human HeLa cells.
A DNA molecule comprising a gene encoding transposase under the control of a suitable promoter.
The transposase containing vector may be transfected into the appropriate cell line and selection applied.
The longest P elements encode transposase needed for transposition.
All transposase ORFs are cloned in identical restriction sites in a CMV expression vector.
The probe used corresponds to the ORF encoding the Impala transposase.
One suitable transposase is the Piggybac transposase.
Sleeping Beauty transposase.
The transposase integrates its adapter load into DNA while fragmenting it.
Nucleic acid encoding me chimeric transposase can take the form of RNA or DNA.
O The transposase gene is flanked by two DNA.
The insertion sequences are named I end and O end which are substrate of the transposase.
The paired subdomains in SB transposase were designated PAL and RED.
Disclosed are compositions, wherein the integrating enzyme is a transposase.
Cloning of the A and B transposase genes of the Mu phage on a multicopy plasmid.
