Examples of 'transwell' in a sentence
Meaning of "transwell"
The noun 'transwell' might refer to a type of cell culture insert used in laboratory experiments or medical research
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- A membrane insert used for cell cultures, between agar wells.
How to use "transwell" in a sentence
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transwell
Transwell plates were cut to produce individual wells.
Neutrophils were incubated in one chamber of a transwell plate.
Such assays employ transwell tissue culture inserts.
Transwell membranes were removed and placed in paraffin blocks.
Apical an basolateral supernatant of transwell plates were used for analysis.
Each transwell was performed in quadruplicate.
Immortalized epithelial cell line cultures were seeded on transwell membranes.
An in vitro transwell migration assay was performed.
A modified version of a previously described transwell migration technique was used.
The control transwell experiments demonstrate this to be a genuine cell-cell interaction.
Experiments were also performed using whole bacteria in a transwell filter system.
The wells contained a transwell insert to allow two compartments per well.
The effects MARCKS knockdown by siRNA on cell migration were determined using a transwell assay.
This chilled solution was filled in transwell culture chambers avoiding air bubbles.
Shown is the relative number of cells having traversed to the undersurface of Transwell chambers.
See also
Scheme of the MDCK transfectants growing on the Transwell membrane inserts as a polarized monolayer.
Membranes from transwell migration chambers were coated with either native or denatured collagen type-IV.
The latter was further suggested by scanning electron microscopy in the Transwell membrane studies.
One day later, transwell inserts containing postpartum cells were added according to the following scheme,.
Migration trial in Transwell.
As a control, a Transwell chamber without addition of bacteria is used.
Antigen-specific suppression was also examined by a transwell assay.
Transwell inserts were placed above the SKIN2 ™ to keep the skin construct submerged.
For example, a commercially available transwell is preferably used.
Human lung microvessel endothelial cells ( HLMVEC ) were grown to confluence on a transwell.
Jurkat T cells were added to the upper compartment of transwell devices with 5 µm pore filters.
The transwell was incubated at 37°C overnight to allow the collagen to solidify.
Figure 1G is a bar graph showing the results of a transwell migration assay.
Cocultures were performed using transwell culture wells with two chambers separated by a 0.45 µm filter.
In the present study, cell migration in response to THC was measured using transwell assays.
Compounds are added to the cells in the transwell inserts at a final concentration of 0.5 % dimethylsulfoxide.
Alternatively, cardiomyocytes can be transfected by exposure to high titer retrovirus in a Transwell coculture system.
Cells are then placed on the Transwell insert that has been pretreated with 0.2 % gelatin.
A ∼ c ; A set of views showing motility of tumor cells examined with a transwell double chamber.
Transwell inserts were then transferred to an anaerobic culture box within the anaerobic workstation at 37°C.
Prior to each experiment the apical surface of each Transwell was washed 3X with PBS.
Alternative transwell method Primary CL1-5 cells originated from human adenocarcinoma lung cancer.
Data are expressed as means CFU per Transwell filter ± SEM.
The transwell migration systems are manufactured by Neuroprobe, Gaithersburg, MD, USA.
Chemo-attraction of peripheral blood mononuclear cells ( pbmc ) was analyzed in a transwell system.
Cell migration assay was performed in Transwell migration chambers ( pore size 8mm ; Costar ).
Figure 8B shows histological staining of the collagen-coated transwell culture.
Chemotaxis assays were conducted using a TransWell chamber ( Corning Costar, Cambridge, MA ).
Sample 5A, The ECF is poured in dilute homogeneous form into a transwell.
Transwell system ( Corning ) was used for co-culture experiments.
Caco-2 cells were cultured on Transwell plates.
B, Transwell suppression assay in which neutralizing antibodies to semaphorin-4a ( Sema4a ) were included.
To this end, MCF10A cells were employed in a transwell assay system.
Additionally, we employed transwell culture system with 0.4µm size ( Sigma ) to plant lymphocytes.
FIG . 15 displays results from the open well and transwell side by side.
