Examples of 'tris buffer' in a sentence
Meaning of "tris buffer"
is a chemical solution commonly used in biological and biochemical laboratories to maintain a stable pH level. It is a mixture composed of three primary components: Tris(hydroxymethyl)aminomethane, an acid, and a conjugate base. This buffer system helps resist changes in pH when adding acids or bases, ensuring that the experiment's conditions remain constant
How to use "tris buffer" in a sentence
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tris buffer
Tris buffer and collect the effluent fraction.
The buffer is an imidazole or tris buffer.
Tris buffer which could react with the ester groups.
The sediments are resuspended in tris buffer.
Tris buffer can also have an auxiliary stabilising effect on the enzymes of the composition.
The labeled oligonucleotide was dialyzed against tris buffer solution.
Tris buffer which might react with the hydrophilic polymers in the next stage.
The reaction mixture was then dialyzed against a tris buffer.
The use of a Tris buffer is preferred.
The reaction is quenched with Tris buffer.
In a variant of this example Tris buffer can be used in place of phosphate buffer.
The reaction was quenched with Tris buffer.
Tris buffer Mixed equal volume of two Tris buffer.
The precipitates were suspended in a Tris buffer solution.
To each tube was added Tris buffer with or without the NADPH generating system.
See also
Disconnected the reservoir from the Tris buffer.
As a control, Tris buffer was used in place of the test compound.
Plates were washed with Tris buffer as before.
Filters were processed by base treatment followed by neutralization in Tris buffer.
The resultant pellet is rehomogenized in Tris buffer and centrifuged again.
The separating column is prepared with a polymer previously swollen and equilibrated in Tris buffer.
The resultant pellet was rehomogenized in Tris buffer and centrifuged again.
This feedstock was loaded onto the immunoadsorbent which was then washed with Tris buffer.
The enzyme activity was decreased as the Tris buffer concentration was increased.
Plates are air dried and bound stain is solubilized with Tris buffer.
The membrane pellet was resuspended in fresh Tris buffer and pelleted again by centrifugation.
The plates are air dried and the bound colorant is dissolved with Tris buffer.
The membrane fraction was reconstituted with Tris buffer for radioligand binding studies.
The cDNA is then ready to use after neutralization by adding Hcl and Tris buffer.
The immunoadsorbent was then washed three times in Tris buffer and packed in a glass column.
A Tris buffer solution can be used as the pH-raising stopping compound.
The control sample consisted of plasma and Tris buffer without calcium.
The Tris buffer suspension is then treated with dithiothreitol ( DTT ) and fumed silica.
The cell mass was again suspended in the aforementioned Tris buffer solution.
Lane 1 - sample extracted in Tris buffer without SDS in acoustic nerve and spiral ganglion.
Connected the reservoir with Tris buffer.
The Tris buffer suspension can then be treated with dithiothreitol ( DTT ) and fumed silica.
The buffer is preferably Tris buffer.
Tris buffer containing,.
The particles were resuspended in Tris buffer containing BSA.
In a preferred embodiment of the invention, the denaturation solution is a Tris buffer.
The resulting pellet was re-homogenized in Tris buffer and centrifuged again.
Appropriate solutions for this purpose are sodium hydroxide, phosphate - or Tris buffer.
After dissection, the forebrains are homogenized in Tris buffer in a Teflon ® glass homogenizer.
In one of the embodiments of the present invention, the buffer is Tris buffer.
Preferably, the antibiotic is present in a Tris buffer when immobilized to the detection zone.
Preferably, the composition includes tris buffer.
The membrane is advantageously equilibrated with a Tris buffer, particularly a Tris buffer having the following characteristics,.
For example phosphate buffer, glycine buffer, borate buffer or tris buffer can be used.
The Tris buffer solution is hereinafter referred to as " Tris-HCl ".
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Examples of using Tris
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Tris does not know what she gave up
Examples of such buffers include tris buffers
Tris is doing his impersonation of the mad conductor
Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
