Examples of 'tris-hcl' in a sentence
Meaning of "tris-hcl"
tris-hcl (noun): A buffer solution frequently used in biochemical research, consisting of tris(hydroxymethyl)aminomethane and hydrochloric acid
How to use "tris-hcl" in a sentence
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tris-hcl
A more preferred buffering agent is Tris-HCl.
The Tris-HCl buffer was added to the pellet and recentrifuged as above two times.
The pellet was washed with a Tris-HCl buffer.
Tris-HCl having a buffering action in a neutral to weakly alkaline range is used.
The fibril precipitate was washed twice with Tris-HCl.
Purified plasmid DNA were diluted in a tris-HCl solution to generate a standard curve.
An acetate buffer or a phosphate buffer may be substituted for the Tris-HCl buffer.
Compound A tris-HCl salt was highly soluble in water.
The enzyme activity demonstrated in Tris-HCl buffer ref.
HEPES and Tris-HCl are commercially available from the Sigma-Aldrich Company Limited.
Between incubations sections were washed with Tris-HCl saline buffered solution.
HEPES is known to be a biologically favorable buffering system as opposed to Tris-HCl.
The precipitate was resuspended in the same Tris-HCl buffer as used above.
The use of a Tris-HCl diafiltration buffer with a pH of 8.5 but of unspecified concentration.
At the same time to another aliquot an identical volume of buffer Tris-HCl was added.
See also
Of the buffers used, Tris-HCl showed the greatest changes over the pH range.
The enzyme can be released by rapidly resuspending the cells in cold Tris-HCl buffer.
The free amines in the Tris-HCL react with the remaining available NHS-esters.
Suitable washing buffers according to the invention comprise but are not limited to Tris-HCl buffers.
Thus, rat brain was homogenized in Tris-HCl buffer and centrifuged.
Cells were pelleted by centrifugation and resuspended in 25 mM Tris-HCl.
The pH of the solution is lowered to 8 with Tris-HCl and incubated with trypsin.
In some embodiments, the elution buffer comprises or is Tris-HCl.
Finally, high concentrations of NaCl or Tris-HCl buffer were found to inhibit PCP 's activity.
The buffering agent can be Tris-HCl.
However, use of tris-HCl or EDTA as buffer increased the analytical assay 's detection ability.
Standard buffer systems like PBS or Tris-HCl can be used.
Tris-HCl buffer is preferably used.
HEPES buffer based media performed better than Tris-HCl buffered media.
Reagents Tris and Tris-HCl ( UltraPure ) were obtained from Life Technologies.
The elution was neutralized using Tris-HCL pH 8.
HEPES and Tris-HCl are commercially available from the Sigma-Aldrich Co. Ltd.
The medium is buffered by tris-HCl.
Most preferably, the buffer is Tris-HCl at a concentration of 25mM.
In some embodiments, the buffering agent is Tris-HCl.
A gelatin-Sepharose 4B column is first prewashed with a Tris-HCl solution in order to equilibrate the gel.
In some instances, the wash buffer comprises or is Tris-HCl.
Suitable elution buffers include Tris-HCl and NaCl with a pH of 8.5.
In some instances, the elution buffer comprises or is Tris-HCl.
The Tris-HCl may be included in a concentration of from about 5 mM to about 20 mM.
An exemplary buffer is Tris-HCL.
More preferably, the buffer is Tris-HCl at a concentration of between 0nM and 50mM.
In some embodiments, the wash buffer comprises or is Tris-HCl.
A suitable amplification buffer contains Tris-HCl as a buffering agent in the range of about 10 to 100 mM.
In some instances, the elution buffer comprises or is Tris-HCl at neutral pH.
Reagents Tris and Tris-HCl ( UltraPure ) were obtained from Gibco-BRL.
In some embodiments, the elution buffer comprises or is Tris-HCl at neutral pH.
TRIS-HCl buffer is also acceptable but gives about 25 % lower activities.
The filters were washed with ice-cold Tris-HCl buffer and dried.
The buffers can be conventional buffers such as acetate, borate, citrate, phosphate, bicarbonate, or Tris-HCl.
