Examples of 'u-pa' in a sentence
Meaning of "u-pa"
U-pa is a noun that may refer to a specific entity or object, but without more context, it is unclear
How to use "u-pa" in a sentence
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u-pa
A buffer for assaying u-PA activity is added.
The u-PA activity is modulated by various interaction of other proteases or inhibitory systems.
Construction of phage display libraries of u-PA variants.
One molecule of u-PA can generate more than one molecule of plasmin.
Chymotrypsin cleavage in the linker region of u-PAR reduces its affinity for u-PA.
The high u-PA level vía this pathway leads to elevated levels of plasmin.
Firstly, the effect of this antibody on u-PA activity in solution was determined.
Both u-PA and streptokinase lack fibrin selectivity and fibrin affinity.
A mixture of phosphorylated and unphosphorylated u-PA can be treated with the phosphorylating enzyme.
Receptor-bound u-PA is catalytically active on the surface of the cell without requiring internalization.
This indicates that the only protein necessary for conversion of plasminogen to angiostatin is u-PA.
In summary the u-PA system seems an ideal starting point for a potential tumor therapy.
The plasmids were used for sequencing the entire u-PA gene using the following sequencing primers.
The u-PA system has received broad attention in the literature, especially in the last decade.
The restriction endonuclease map of the human u-PA cDNA insert is shown in Fig.
See also
Screening of u-PA phages with increased reactivity and catalytic efficiency towards new substrate sequences.
Based on the known structure of genomic u-PA DNA A.
Expression of modified u-PA enzymes by transient transfection of COS cells.
Chromozyme U is a direct substrate for u-PA.
The human u-PA receptor has been purified and characterized Behrendt et al.
In addition, a specific receptor has been identified for u-PA.
The eluate of phosphorylated u-PA free of unphosphorylated u-PA is collected.
Under physiological conditions this cleavage is catalysed by t-PA or u-PA.
Fragments of u-PA containing only the receptor binding domain, e . g.
The purified protein could be chemically cross-linked with u-PA.
Construction of mutant u-PA and phage display libraries of mutant u-PA.
Cleavage results in the formation of the two-chain active u-PA.
Therefore, phosphorylated u-PA only represents part of the total u-PA.
This reaction is catalysed by t-PA or by u-PA.
Inhibition of u-PA or u-PA variant phage also was assessed.
The phagemid was used for monovalent display of wild type or mutant u-PA ( see below ).
The nucleotide sequences of u-PA cDNA and t-PA cDNA are known cf.
Fig . 2 illustrates the nucleotide sequence and deduced amino acid sequence of human u-PA cDNA.
Selection of variant u-PA from u-PA phage libraries against mutant inhibitor.
This fragment contains the pCS16 vector and coding sequence for the u-PA growth factor domain.
Therefore the anti-u-PAR antibody gave no specific inhibition of u-PA activity.
Unlabelled catching antibody, monoclonal antibody reacting with the B-chain of u-PA.
Therefore, biosynthetic pro-u-PA and u-PA are shielded from the action of the inhibitors.
EXAMPLE 2 Construction of phage display libraries of u-PA variants.
Measuring the u-PA activity of the cell culture supernatant of said cell of step ( d ).
Fig . 1 is a restriction endonuclease map of human u-PA cDNA.
For control experiments, wild-type u-PA phage was assessed under the same conditions.
Under normal conditions, the endothelial cells contain t-PA . but no u-PA.
T-PA and u-PA are functionally and immunologically distinguishable.
Cloning of wild-type u-PA into phagemid.
In one example, variant u-PA polypeptides were identified against selection of a modified PAI-1 polypeptide.
Fig . 17 schematically depicts the construction of u-PA expression plasmid pBR3a.
This fragment contains the u-PA G-domain and the u-PA B-chain connected to the vector molecule.
A 1.3 kb SmaI-BamHI fragment comprising most of the u-PA coding sequence is isolated.
Single-chain u-PA can be represented by the following formula,.
