Examples of 'using restriction' in a sentence
Meaning of "using restriction"
using restriction: This phrase suggests employing limitations or constraints in a particular context. It implies that some form of control or regulation is being applied to restrict certain actions or behaviors
How to use "using restriction" in a sentence
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using restriction
Deletion can be effected using restriction endonucleases.
Using restriction enzyme digestion.
Its structure was confirmed using restriction enzymes.
Using restriction ring.
The isolated plasmid was analyzed using restriction enzymes.
Using restriction Continuous working.
The correct orientation was confirmed using restriction digests.
Using restriction enzymes, we analyzed DNA fragments… which vary with each individual.
A fragment within the gene is removed using restriction endonuclease.
Enrichment was monitored using restriction fragment length polymorphism ( RFLP ) and flow cytometry using live cells.
The preliminary screening of the positive clones was carried out using restriction digestions.
Verification of the cloning was done first using restriction enzyme digestion and subsequently by sequencing.
Recombinants containing cDNA inserts in the correct orientation are be selected using restriction mapping analysis.
They can then be excised using restriction enzymes and cloned into a vector.
The ability to incorporate a gene sequence into a suitable vector is typically performed using restriction endonucleases.
See also
The product is amplified using restriction sites contained in the PCR primers.
Such a technique is illustrated in Example using restriction enzymes.
This can be done for example using restriction fragment-length polymorphism ( RFLP ) to identify an individual.
These sequences can be produced by PCR or using restriction enzymes.
DNA was digested using restriction enzymes ( New England Biolabs, Beverly, MA ) according to manufacturers ' recommendations.
The jnb amplification products were subjected to rflp using restriction endonucleases drai and hinfi.
In some cases, enzymatic digestion may be performed using enzymes such as using restriction enzymes.
AFIP templates were prepared using restriction enzyme combination EcoRI / MseI.
The excision of the said DNA fragment may be performed using restriction enzymes.
This PCR fragment was then digested using restriction enzymes BsaI and NheI.
That said DNA was the desired one which was confirmed using restriction enzymes.
The exon 2 polymorphism was detected using restriction enzyme digestion with BsmA I.
Dephosphorylation can play a key role in molecular biology, particularly cloning using restriction enzymes.
The obtained PCR products were digested using restriction enzymes NcoI and BamHI.
The genetic information for EF on the recombinant bacteriophages was localized using restriction enzyme analysis.
The correct insertion of the fragment was confirmed using restriction enzyme analysis and DNA sequencing.
In particular embodiment of the present invention, detection is accomplished by using restriction endonucleases.
In addition, fragments could be readily generated using restriction enzyme digestion and / or ligation.
The plasmids are isolated from the clones selected on ampicillin+avidin and analyzed using restriction enzymes.
A B-Gal gene fragment was cut from pLMBL using restriction enzymes BlnI and BglII.
Excision of a portion of the mature t-PA DNA may be effected by using restriction enzymes.
Alternatively, Entry Vectors were produced using restriction endonucleases and ligases.
Colonies were picked, and plasmid DNA was purified and analyzed using restriction enzymes.
The purified PCR DNA was disgested using restriction enzymes, NdeI and PstI.
Plasmids having acquired the XbaI-linker sequence are detected by digestion using restriction endonuclease XbaI.
A B-Gal gene fragment was cut from pZeo / lacZ using restriction enzymes PstI and XhoI.
Plasmid DNA from single colonies was purified and analyzed using restriction endonucleases.
The rep gene was then deleted from plasmid pVD88 using restriction enzymes RsrII and XbaI.
According to Morinaga et al ., a fragment within the gene is removed using restriction endonuclease.
Plasmid pSGsetrapK, was isolated and the construct was verified using restriction digests and sequence analysis.
Florin ( Cupressaceae ) controlled crosses was demonstrated using restriction fragment length polymorphisms.
Figure 6 is an autoradiogram of PAGE analysis of amplification reaction using restriction fragments as template.
An Act promoter fragment was cut from pBlue / Act using restriction enzymes SalI and BlnI.
An RSV promoter fragment was cut from pBlue / RSV using restriction enzymes XhoI and PstI.
Fifty individual recombinases were functionally analysed using restriction analysis in E. coli ( data not shown ).
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