Examples of 'using reverse transcriptase' in a sentence
Meaning of "using reverse transcriptase"
using reverse transcriptase: A process that involves the enzyme reverse transcriptase to create complementary DNA (cDNA) from an RNA template, commonly used in molecular biology and biotechnology
How to use "using reverse transcriptase" in a sentence
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using reverse transcriptase
The reaction using reverse transcriptase.
The obtained mRNA is used to synthesize cDNA using reverse transcriptase.
Using reverse transcriptase the human lymphocyte mRNA was converted to single-stranded cDNA.
The samples were normalized prior to assay using reverse transcriptase activity.
CDNA was prepared using reverse transcriptase ( reverse transcriptase ) with poly A+primers on RNA.
B cell mRNA sequences encoding human antibodies are used to generate cDNA using reverse transcriptase.
CDNA can then be synthesized using reverse transcriptase from the obtained mRNA.
The mRNA obtained from human tissue or cells is converted into cDNA using reverse transcriptase.
CDNA was synthesised using reverse transcriptase primed by oligo ( dT ).
Synthesis of HCV cDNA from the samples was accomplished using reverse transcriptase.
Next, cDNA is synthesized using reverse transcriptase with poly ( A ) RNA, collected above, as a template.
CDNA copies of the mRNA are produced using reverse transcriptase.
Hepadnaviruses replicate through an RNA intermediate which they transcribe back into cDNA using reverse transcriptase.
RNA may be amplified by using reverse transcriptase PCR.
From the obtained mRNAs, antibody variable region-encoding cDNAs can be synthesized using reverse transcriptase.
See also
The mRNA is converted to cDNA by using reverse transcriptase ( See Example 2 ).
Particularly important vectors allow cloning of cDNA ( complementary DNA ) from mRNAs produced using reverse transcriptase.
Purified viral RNA was reverse transcribed into cDNA using reverse transcriptase and random hexamer primers.
CDNAs of the antibody V regions are synthesized from the obtained mRNAs using reverse transcriptase.
Poly ( A ) + mRNA fragments were converted to RNA-cDNA hybrids using reverse transcriptase primed with random hexanucleotides.
These poly A+RNAs serve as template for reverse transcription reactions using reverse transcriptase.
Reverse transcribe RNA to cDNA using reverse transcriptase.
CDNA is synthesized from the thus obtained mRNA using reverse transcriptase.
Complementary DNA ( cDNA ) was synthesized from total RNA using reverse transcriptase primed with oligo dT.
A double-stranded cDNA was synthesized from human lung-derived polyA RNA using reverse transcriptase.
The RNA obtained was transcribed using reverse transcriptase.
Next, cDNAs are synthesized from the resulting mRNAs using reverse transcriptase.
Add 3 C bases to 3 ′ end of target using reverse transcriptase.
Vectors also allow cloning of cDNA ( complementary DNA ) from mRNAs produced using reverse transcriptase.
CDNA ( complementary DNA ) is usually prepared from mRNA using reverse transcriptase.
The RNA is then converted to complementary DNA ( cDNA ) using reverse transcriptase.
As DHPLC works on DNA, mRNA samples are converted into cDNA using reverse transcriptase.
A cDNA library was prepared from the poly ( A+ ) mRNA using reverse transcriptase.
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