Examples of 'using trypsin' in a sentence
Meaning of "using trypsin"
using trypsin: This phrase refers to the action of employing trypsin, an enzyme that catalyzes the breakdown of proteins into smaller peptides. It is commonly used in biochemical experiments for protein digestion
How to use "using trypsin" in a sentence
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using trypsin
These bands are hydrolyzed using trypsin.
The cells were detached using trypsin treatment immediately before the cell transplantation.
Cells were passaged by using trypsin.
The aHNSC were split using trypsin and EDTA under standard conditions.
Cells were cultured and passaged into tissue culture flasks using trypsin.
Transfer is accompanied by enzymatic dissociation using trypsin in order to produce multiple colonies.
The enzyme is activated by the enzymatic removal of the pro domain using trypsin.
The HSC were split using trypsin and EDTA under standard conditions.
The adherent cell monolayers were washed once with PBS and then detached using trypsin.
Subculture is carried out using trypsin and EDTA.
It can be obtained from as1 casein by enzymatic hydrolysis, in particular by using trypsin.
The cell lines were harvested using trypsin and washed 3x in maintenance media.
Preferably, protein hydrolysis is performed using trypsin.
Enzymatic treatment of Glatiramer acetate using trypsin to obtain peptide fragments of short chain lengths ;.
Optionally, the enzymatic dissociation is performed using trypsin.
See also
Then, MSCs were collected using trypsin and diluted 20 fold with the medium.
In some methods, the enzymatic dissociation is performed using trypsin.
Cells were harvested using trypsin and a Tomtec 96 well plate cell harvester.
Cells growing exponentially were harvested using Trypsin EDAT solution.
Subculturing was performed using trypsin when 60-80 % confluence was obtained as specified by the supplier.
Protein bands were excised and digested in-matrix using trypsin.
The conversion reaction can be followed using trypsin on a nondenaturing polyacrylamide gel ( FIG . 8 ).
In one example, enzymatic dissociation can be performed using trypsin.
CHO Flp-In pLL113 cells were harvested using trypsin and washed 3x in maintenance media.
In some embodiments of the method, enzyme proteolysis is performed at step b by using trypsin.
In some other embodiments, step b1 is performed by using trypsin as the sole added protease.
In some populations or in vitro cultures, the enzymatic dissociation is performed using trypsin.
Cells adherent to the polypeptide are also retrieved using trypsin or cell dissociation buffer e . g.
To obtain an active enzyme, the pro-kallikrein produced was activated in vitro using trypsin.
Example 4 Trypsin Peptide Mapping The digestion of the sample using trypsin is described in Example 3.
The proteins in the depleted serum / plasma sample are digested into peptides using trypsin.
The cells were passaged using Trypsin ( Gibco ).
When the flasks approached confluence ( approximately 3 to 4 days ) the cells were detached using trypsin.
In the most preferred embodiment, pretreatment is achieved using trypsin and lysolecithin.
The cells were sequentially washed with PBS, PBS containing 10 mM EDTA, then removed using trypsin.
The preparations obtained from step 4 can be dissociated into single cells using trypsin and DNase.
The expanding NSC population was passaged by mild enzymatic treatment, such as using trypsin.
The expanding NSC population is passaged by mild enzymatic treatment, such as using trypsin.
Cells were grown in the presence of drug for 48 hours, and harvested using trypsin.
According to this known method, the cells 1 are harvested by enzymatic treatment using Trypsin ( I ).
Methods, Basal forebrain cultures were prepared from E17 ( Embryo Day 17 ) embryonic rats using trypsin dissociation.
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