Examples of 'vector backbone' in a sentence
Meaning of "vector backbone"
vector backbone ~ a foundational structure or framework used for cloning and expressing genes in molecular biology
How to use "vector backbone" in a sentence
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vector backbone
The vector backbone was then purified on an agarose gel.
Plasmids were digested with PacI to remove vector backbone.
The vector backbone is a pUC derived plasmid in all cases.
Basic expression vector backbone.
Inserted vector backbone sequences are subsequently removed by the action of an appropriate recombinase.
They are then ligated into the desired vector backbone.
These may serve as vector backbone for the constructs.
Taqman analysis was used to determine presence or absence of vector backbone sequences.
These may serve as vector backbone for the constructs of the present invention.
Only those clones will survive that have lost the vector backbone comprising the SacB gene.
Thus, only part of the vector backbone sequence is transferred to the eukaryotic nucleus.
Plasmids were digested with Pad to remove vector backbone.
Sequence of the vector backbone - cDNA junction.
The final fully assembled sequence was carried in a BAC with pBeloBAC as the vector backbone.
Generally this is based on the vector backbone and the desired result.
See also
The second DNA fragment is then purified away from its linearized vector backbone DNA.
In principle, any vector backbone can be used.
The linear fragment used for transformations was digested out from the vector backbone with Xba I.
Can be used for the vector backbone and cassette sequences,.
This removed one of the two Pvu I and Pvu II sites in the vector backbone.
Optionally, the resulting vector backbone was purified by agarose gel-electrophoresis and subsequent gel extraction.
The exemplary virus used essentially comprised the adenovirus vector backbone disclosed by Wills et al.
Vector backbone integration is also likely to interfere with T-DNA tagging experiments.
Preferably, the expression vector backbone is pTTO.
The non-lethal genes provide a visual means to distinguish transgenic events that contain the vector backbone.
The Pee1/Bgl II dephoshorylated vector backbone was then purified on an agarose gel.
Within the expression cassette ( s ) or the vector backbone.
The desired vector backbone fragment was purified using Qiagen 's QIAquick gel extraction kit.
The yeast sequence was then removed from the vector backbone of pCHAN1.
Into this vector backbone two PCR-derived DNA fragments will be cloned in a three-way ligation reaction.
Since then, one has tried to understand the mechanism of vector backbone integration.
Then the vector backbone fragment was filled in with T4 polymerase followed by ligation.
The expression cassettes were released from the E. coli DNA vector backbone by NotI restriction.
The vector backbone is pTrcHis2B.
More than 80 % of the adenovirus viral genome remains in the vector backbone.
The generated expression vector backbone was designated pVAX10.
A vector backbone comprising:.
The vector may be based on or derivable from the MP71 vector backbone.
The expression vector backbone ( mock ) was used as negative control.
All the receptors were cloned from rat tissue into the PCR3.1 vector backbone.
Plasmid 6 comprises p43 vector backbone containing the AAV-ITRs and encoding wild type Gag.
Primer J46 was designed to hybridise within the B-lactamase gene within the vector backbone.
The vector backbone was re-ligated and transformed into E. coli.
The 3 ' oligonucleotide overlapped the Bcl I site in the vector backbone.
The pTZ57R/T vector backbone is represented by the fragment observed at approximately 5.4Kb band position.
Figure 8, ( A ) Schematic representation of human FVIII variants designed and cloned into a SIN lentiviral vector backbone.
The 4.5 kb gel-purified fragment of the vector backbone was used for ligation.
Of course, vector backbone sequences may separate the expression cassettes ( MASM ) and ( POI ).
The 9.4 kb pALK193 expression cassette was cut from the vector backbone by Eco RI restriction.
In addition the vector backbone contains a 5 ' neo gene and a 3 ' WPRE.
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Examples of using Backbone
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Just grow a backbone and call her
Backbone lends support but does not involve
Public administration is the backbone of effective government
