Examples of 'was denatured' in a sentence
Meaning of "was denatured"
was denatured: this phrase indicates that a protein or compound lost its natural structure and function due to external factors such as heat or chemicals
How to use "was denatured" in a sentence
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was denatured
The amplification product was denatured and allowed to renature.
DNA was denatured as per standard protocol.
The preferred solvent used in the formulation was denatured alcohol.
The probe was denatured prior to loading on the denatured chromosomal slides.
Endo H digestion The protein was denatured as described above.
The probe was denatured and could be stored frozen for at least five weeks.
But it was definitely his . It was denatured.
Insulin without any additive was denatured and the band was almost gone in native gel.
One base of the resulting overhang was filled using biotin-dATP, and the DNA was denatured.
The PCR product was denatured and made single stranded.
From each enzyme stock solution, a part was denatured by boiling for 15 minutes.
The RNA was denatured by glyoxal treatment and transferred to a nylon membrane.
Following that the sample was denatured by boiling for 5 minutes.
Then, TdT was denatured and inactivated by the addition of a phenol-chloroform mixture.
After the first detection, the surface was denatured with 0.2 M sodium hydroxide for 1 min.
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DNA was denatured by alkaline denaturation in 0.2 N NaOH prior to the sequencing reactions.
The cycling conditions were as follows, the DNA was denatured at 94 degrees C for 2.5 minutes.
The target DNA was denatured and reactions were amplified using the liquid enzyme preparation.
DNA on the blots was denatured and prepared by standard methods.
RNA was denatured and electrophoresed through a 1 % agarose-formaldehyde gel and electrotransferred to Zeta Probe.
Following sonication, the DNA was denatured by being boiled in water bath for 5 minutes.
The probe was denatured by heating to 95°C for 5 minutes and quenching on ice.
After separation, the DNA was denatured by standard methods Sambrook et al.
The protein was denatured with B-mercaptoethanol ( B-mer ) or with sodium dodecyl sulfate ( SDS ).
The duplex DNA was denatured as described above.
The purified RNA was denatured and separated on a glyoxal agarose gel and urea-PAGE, respectively.
The DNA on the filters was denatured and baked as described above in Example 3.
The filter was denatured and rehybridised with ABL and B-actin.
Briefly, the reaction mixture was denatured by heating at 95°C upon completing the reaction.
Collagen was denatured by boiling for 15 minutes.
Before use, the probe was denatured at 96°C and immediately put on ice.
The template was denatured by an initial five minute incubation at 96°C.
The insoluble material was denatured and " refolded " to reconstitute biologically active leptin.
After electrophoresis, the DNA was denatured and transferred to a nitrocellulose membrane as described in example 3.3.
Precursor human IL-1B was denatured by boiling a solution of precursor IL-1B for fifteen minutes.
The probe was denatured by incubating at 95°C for 5 minutes before use.
Each sample was denatured at 65 °C for 5 minutes and then placed on ice.
The probe solution was denatured by heating to 95°C for 10 minutes and added to the filters.
The solution was denatured at 95 ° C for 6 min and applied to the Surecell well.
The cDNA riboprobe was denatured at 100 degrees C for 5 minutes and placed on ice.
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Examples of using Denatured
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Denatured milk proteins and mucoid saliva
Products containing denatured medium mineral oils
Denatured or soot free alcohol is used as fuel
