Examples of 'were subcultured' in a sentence
Meaning of "were subcultured"
were subcultured: the act of transferring a small sample of cells or organisms into a new culture medium to allow for further growth and study
How to use "were subcultured" in a sentence
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were subcultured
The cells were subcultured twice prior to treatment.
Fourteen clones from each transformation were subcultured.
All cells were subcultured once a week.
When actively growing, the cells were subcultured.
The cells were subcultured when they reached confluence.
Suspected Helicobacter pylori colonies were subcultured onto fresh medium.
Cells were subcultured before reaching confluence.
The receptor stably expressing cells were subcultured in respective above media.
Explants were subcultured to fresh medium every two weeks.
At two-weekly intervals all calli were subcultured on to fresh medium.
Some wells were subcultured from big wells to smaller ones.
Prior to use in the experiments, bacteria were subcultured at least three times.
Cells were subcultured cell culture flasks as they reached confluence.
The obtained transformed plants were subcultured by sterile in vitro culture.
They were subcultured using standard tissue culture techniques for adherent cells.
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Clones producing antibodies reactive to PcrV were subcultured to larger culture dishes and then isotyped.
They were subcultured on YPD medium so as to obtain isolated colonies.
In the absence thereof, cells were subcultured in different flasks.
Calli were subcultured to fresh medium every 4 weeks.
For phosphorylation studies, cells were subcultured in 6-well plates.
The BMMCs were subcultured every week with fresh medium.
Each concentration solution in 3 replicates, the cells were subcultured 48 hours after dosing.
The cells were subcultured twice a week.
The medium was changed every 48 h and cells were subcultured at 90 % confluency.
Germinating embryos were subcultured to fresh medium until rooted shoots were obtained.
Cells were maintained at 37 degrees Celsius with 5 % CO2 and were subcultured weekly.
Stock strains were subcultured twice to ensure purity and viability prior to testing.
After a total of 30 days in EM culture, cells were subcultured as follows in Example 2.
These colonies were subcultured onto further plates where the same selection criteria were applied.
Suspension cultures were subcultured weekly in N6 medium.
They were subcultured on new medium every 3 weeks.
FreeStyle 293F cells ( Invitrogen Corp. ) were subcultured and cultured according to the manual.
They were subcultured with intervals of 3 days.
Prior to testing, all isolates were subcultured twice onto enriched brucella agar plates.
The cultures were subcultured the following day, and the subcultures were again incubated overnight.
The following morning isolated colonies were subcultured onto fresh BAPs and incubated at 35°C overnight.
Cells were subcultured or harvested when cultures reached approximately 85 % confluence.
During a 6-week period, blood cultures were subcultured in broth during the first 18 h of incubation.
Broths were subcultured onto the three media ( 10 µl of broth per plate as described above ).
After 2 days the cultures were subcultured to perform a batch culture for a period of 5 days.
Cells were subcultured 1:4 once a week and fed fresh medium 3 times a week.
Then, the neural stem cells were subcultured while the proliferation thereof was observed.
Cells were subcultured weekly into fresh medium using a 4 % inoculum.
After the growth, the cells were subcultured according to the different described techniques.
Flasks were subcultured by dilution with fresh medium back to 2 x 105 cells / ml at 5 day intervals.
Proliferating cells were subcultured 5 times on fresh STO feeders.
These were subcultured onto DST nutrient medium and incubated at 37°C for 18 hours.
The transformant colonies were subcultured to fresh agar containing 5 µg / ml hygromycin B.
The cells were subcultured and washed twice with cold PBS.
Since then the cultures were subcultured three times in 250 mL spinner flasks.
