Examples of 'x-gal' in a sentence
Meaning of "x-gal"
X-Gal: An abbreviation for '5-bromo-4-chloro-3-indolyl beta-D-galactoside', often used in enzymatic assays and molecular biology research as a substrate for beta-galactosidase
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- 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, used to detect β-galactosidase.
How to use "x-gal" in a sentence
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x-gal
X-gal staining was performed overnight at room temperature.
The explants were then stained with X-Gal stain.
Iodide X-Gal was further functionalized vía incorporation of a histidine tag.
Yeast transformants were plated on media containing the indicator X-Gal.
X-gal staining of tumor cells grown in vitro and in vivo.
Complementation tests between different doa mutants were done on X-Gal plates.
These were stained with X-gal solution after mild fixation.
Transfection efficiency was calculated based upon the percent of X-Gal stained cells.
Extensive X-gal staining was seen in a metastasis found in a thoracic lymph node.
Figure 4 shows faint expression of this protein in an early heterozygous embryo stained with X-Gal.
Double heterozygotes were subsequently analyzed by X-gal staining at 4 weeks of age.
All plaques obtained contained inclusion bodies and stained blue in the presence of X-Gal.
Strong transgene expression was revealed by X-gal staining as described above.
Transformed cells were selected on medium supplemented with X-gal.
The fraction of transgenic embryos with X-gal staining of fetal liver is reported.
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Recombinant viruses were identified by plaques staining positive with X-gal.
On day four an X-gal staining procedure on the transduced cells is carried out.
Tumors were then placed overnight in X-gal solution.
After appearance of plaques, X-gal staining solution is added to check for LacZ expression.
Recombinant plaques were detected after X-gal staining.
The cells are poured on X-gal plates and colonies forming white plaques are obtained.
Some amnionic cells were also X-gal positive.
X-Gal is a chromogenic substrate that produces a rich blue color when cleaved by lactase.
More urothelial cells were stained with X-gal with time.
DNA synthesis activity in X-gal positive cells was then determined.
A plaque which was stained blue in the presence of X-gal was collected.
No X-gal staining was detected in the acinar or islet cells FIG.
Only the tumor cells stained positive with X-gal.
The cryo-sections were then stained with X-gal following the procedure used for the cell lines.
Response of T-cells is expressed as percentage of maximum response using x-gal assay.
X-Gal is preferred for solidified cultures and ONPG for liquid cultures.
B-Galactosidase phenotype of the GlsR clones was determined by X-Gal histochemical staining.
X-gal staining was performed as previously described1.
Cells infected with LacZ vectors were also stained with X-gal.
Resultant viral plaques are screened under an X-gal overlay for expression of B-galactosidase.
Clones were selected on LB agar plates supplemented with carbenicillin and X-Gal.
Cytospins were stained with X-gal and counterstained with Nuclear Red.
Several months later, a portion of the transduced hepatocytes remain X-gal positive.
Prepare the X-Gal mix with.
Its expression is detected by a blue coloration in the presence of the substrate X-gal.
X-gal staining / green fluorescent plaques were selected and further purified.
Parallel cultures were immediately fixed after mock-treatments and stained with X-gal.
A, X-Gal stained reporter cell culture upon transfection with pBluescript.
Transfection efficiency was measured by counting the percentage of cells stained blue with X-gal.
The same results were obtained in an X-Gal plate assay ( data not shown ).
All cells of this type produce blue clones on Petri plates containing X-gal medium.
Figure 1 shows X-gal staining of photochemically transduced WiDr cells.
This screening was carried out in the presence of IPTG and the X-Gal enzyme substrate.
Figure 4 shows X-Gal staining in muscles electroporated with Wnt7a constructs.
This enzyme will give rise to a blue staining when subjected to the substrate X-gal.
