Examples of 'xba' in a sentence
Meaning of "xba"
XBA is a noun that may refer to an abbreviation or a specific term in a particular context
How to use "xba" in a sentence
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xba
A single Xba i site is present at the site of the deletion.
The linear fragment used for transformations was digested out from the vector backbone with Xba I.
Xba I sites were introduced at the both ends of the cassette vía the primers.
The plasmid contains a unique Xba I restriction site located at the site of the deletion.
The vector had no recognition sites for the enzyme Xba I.
The restriction endonuclease cleavage site Xba I is preferred for this purpose.
The nucleotides TCTAGA at the beginning and end of the gene are Xba I sites.
Restriction endonuclease mapping with Pst 1 and Xba 1 confirmed the identity of this material.
These fragments were removed by restriction endonuclease digestion with Hin dIII and Xba I.
The resulting PCR products had Xba l sites incorporated into the 5 ' ends to facilitate cloning.
The underlined sequences indicate the recognition sites for the restriction enzymes Eco RI and Xba I.
Therefore, the Xba i site was converted to a Bam HI site.
The sequence also incorporates suitable restriction sites to assist in cloning, in particular Xba 1 sites.
The recipient vector-fragment was prepared by Xba I digestion and purified from an agarose gel.
This fragment was in turn used as a probe to identify a 4 kb Xba I fragment.
See also
To facilitate cloning, Xba I and Eco RI restriction sites were incorporated at the ends.
Digesting pUCBCPsB with restriction endonucleases Eco RI and Xba I and purifying a resulting large fragment ;.
The Xba I - Bam HI vector fragment was isolated from pLS1 as the first ligation fragment.
Plasmid pTRKH4 was digested with Xba I and subsequently blunt ended with the Klenow fragment.
Xba I and Bam HI were the restriction sites chosen for the cloning of Pcbh1-fobxl cassette.
The purified PCR products were cloned into the Xba I site of the donor plasmid, pFastBac1.
Xba 1 and EcoR 1 sites are thus introduced in the ends of the PCR fragment.
This primer contains an Xba site ( underlined ).
The oligonucleotide was ligated in patag4, after cutting with Eco RI en Xba i.
Digestion with Xba produced 8 fragments.
The 3 ' ( reverse ) primer contained sequences that introduced a stop codon followed by a Xba I restriction site,.
A Hind III - Xba 1 fragment from this clone was then subcloned into the pRc / RSV vector.
These primers contain non-annealing extensions which include Xba I restriction sites ( underlined ).
Each 10 µg of the chromosomal DNAs was completely digested with Eco RV and Xba I.
This fragment was digested with Xba I and then cloned using pCEP4 plasmid ( Invitrogen ).
Before the in vitro transcription, the plasmid was linearized with Xba I ( Promega ).
Cho, unpublished with the Xba I / Sac I fragment containing wtrxh coding sequence from the pWTRXh 1.
In the case of pUC18, the plasmid is cleaved with Hind III and Xba I.
Lane 9 is the result of incubating pUC18 cut with Xba I in the reaction mixture without P2.
After the fill-in, plasmid DNA is digested with the second enzyme, Xba I.
Xba is in the ADH fragment ( 4 bases from its 3 ' end ) and is also not unique.
All the reverse primers complementary to the 3 ' - end, used herein contain an Xba 1 site.
This fragment was cloned into the Xba I site resulting in plasmid pCGN1215.
These clones were screened for orientation using Sac 1 and Sac 1 + Xba 1.
The SmaI site at the 5 ' end, and the Xba I site at the 3 ' end are underlined.
Xba file extension ( OpenOffice . org Basic Module File ).
The TPl1 terminator was removed from plasmid p270 as an Xba I-Bam HI fragment.
The full-length cDNA was cleaved with the Xba I enzyme, and then inserted into pCDNA3 ( INVITROGEN ).
Four oligonucleotides are synthesized, hybridized and ligated to form a 55 base-pair ( bp ) Ava I - Xba I linker.
Zem219a was digested with Bgl II and Xba I, and the vector fragment was recovered.
The 5 ' primers contain a Sac I restriction site and the 3 ' primers contain a Xba I restriction . site.
The purified fragments were digested with EcoR I and Xba I and cloned into the plasmid pKUN19 ( 24 ).
