Examples of 'zeocin' in a sentence
Meaning of "zeocin"
Zeocin is a verb that refers to the action of treating cells or organisms with the antibiotic Zeocin
Show more definitions
- A particular broad-spectrum antibiotic.
How to use "zeocin" in a sentence
Basic
Advanced
zeocin
Zeocin was thus used for transformation selection.
Transformants were selected based on their resistance to zeocin.
This plasmid also encoded a zeocin resistance gene.
This is manifested by robust growth in the presence of zeocin.
The resistance to zeocin allowed the selection of transfected clones.
The selection system of these vectors is zeocin resistance.
Each zeocin resistant cell line was also checked for glucose uptake.
A stable cell line was then generated by zeocin selection.
The zeocin selection system may also be employed in the present invention.
All fermentation was also done without the presence of zeocin.
Expression vectors containing the zeocin drug selection gene are also contemplated.
Selection can be based on resistance to zeocin.
Zeocin is an antibiotic that breaks chromosomal DNA in cells.
The antibiotic resistance gene can be a zeocin resistance gene.
Zeocin was obtained from CAYLA.
See also
Selection was based on resistance to zeocin.
Further described is a Zeocin resistance gene.
Pastoris strain was transformed with the recombinant plasmid and colonies were selected by zeocin resistance.
Hence, the antibiotic zeocin is a preferred selection marker.
Cells incorporating the luciferase reporter gene are selected by including zeocin in the culture medium.
At certain concentrations, zeocin can kill most treated cells, but some cells still survived.
There have recently been developed also the genes for affording resistance to zeocin or hygromycin.
In certain exemplary embodiments, zeocin is used as the selection agent.
The cells were maintained in a medium containing Zeocin.
One day after isolation of the colonies zeocin is added to the medium.
The transformants were selected on YPDS medium containing zeocin.
Insect cell line protein expression vectors containing a Zeocin resistance gene shuttle cassette.
Successful integration events were then selected for with Zeocin.
Transformant clones were selected for increased zeocin production and from dot-blotting.
Positives clones were selected on semisolid LB media containing tetracycline and zeocin.
The copper-chelated form of zeocin is inactive.
Zeocin is blue in colour due to the presence of copper ion Cu2.
Stably transfected cells were selected by Zeocin resistance.
The Zeocin selection marker may be replaced in the expressing vectors by,.
Transformants may be selected by Zeocin resistance.
The Zeocin resistance gene is under the control of the OpIE1 promoter.
Permanently transfected cells are selected with Zeocin and propagated.
The Zeocin gene, present between the cloning sites, maintains selection and stability.
The arrow heads indicate the size of the Zeocin resistance gene.
Individual Zeocin resistant clones were not isolated ; Zeocin-resistant cells were maintained as polyclonal cultures.
A number of potential difficulties are associated with the use of Zeocin as a selectable marker.
All four colonies exhibited zeocin sensitivity and had lost the ability to produce L-lactic acid.
High salt concentrations may inactivate Zeocin.
The transformants were selected under Zeocin ( pPICZalpha ) or histine deficient plates.
For example, the antibiotic may be zeocin.
Four colonies were picked and tested for zeocin sensitivity and lack of L-lactic acid production.
Marker genes being functional in mammalian cells, such as neomycin or zeocin resistance genes ;.
An expression cassette conferring zeocin resistance to Escherichia coli and Pichia pastoris ;.
In some embodiments, provided isolated nucleic acid molecules further comprise a zeocin resistance gene.
Cells resistant to Zeocin and Blasticidin S were then single-cell-subcloned by limiting dilution.
