Examples of 'agarose beads' in a sentence
Meaning of "agarose beads"
agarose beads - Agarose beads are a type of material commonly used in molecular biology for various purposes such as protein purification, DNA electrophoresis, and chromatography. These beads are made from agarose, a polysaccharide derived from seaweed, and have a porous structure that allows them to interact selectively with biomolecules based on size and charge
How to use "agarose beads" in a sentence
Basic
Advanced
agarose beads
This may be immobilized on glutathione agarose beads.
Approaches for preparing agarose beads are discussed herein above.
Method and apparatus for making porous agarose beads.
The agarose beads are then precipitated and washed four times with binding buffer.
The library may be immobilized on for example sepharose or agarose beads.
Background of the Invention Agarose beads are the most common base matrix for chromatography media.
Microdroplet encapsulation involves complete encapsulation of single cells in agarose beads.
Another example of the application of homogeneous agarose beads is affinity chromatography.
The experimental conditions are identical to those used for the experiments on agarose beads.
Lysate was added to equilibrated Ni-NTA agarose beads for affinity purification on a column.
The present invention relates to a process and apparatus for making porous agarose beads.
For instance, a column filled with agarose beads having antibody attached to them.
Sepharose ® is the commonly used name for agarose beads.
The quartz containing agarose beads were washed, wet sieved and crosslinked as above.
The emulsion is then cooled to cause the agarose beads to gel.
See also
Anti-mouse IgG agarose beads were used for immunoaffinity purification of fusión proteins.
The complexes were precipitated with streptavidin-coated agarose beads.
One application of the homogeneous agarose beads is for use in packed-bed liquid chromatography.
The secreted proteins were purified using Ni-NTA agarose beads.
Separating the coated agarose beads of step ( k ) from the liquid of the fourth bath.
It is also widely utilized coupled to magnetic, latex and agarose beads.
The agarose beads were washed extensively to remove the unbound JNK1.
In another embodiment, the solid support comprises porous agarose beads.
Spacers bound to agarose beads are commercially available from, for example, Pharmacia.
In particular, the packing particles may comprise glass, silica or agarose beads.
Streptavidin coated agarose beads ( BRL ) may also be used for this affinity purification.
In a preferred assay method, the polypeptide is immobilised on beads such as agarose beads.
Suitable spacer sequences bound to agarose beads are commercially available from, for example, Pharmacia.
In the most preferred embodiment, the solid support is porous agarose beads.
Fusion protein concentration on the agarose beads was determined by the Bradford protein assay ( Biorad ).
This purified anti-E2T was conjugated with horseradish peroxidase and to agarose beads.
Alternatively, avidin-coated agarose beads can be used.
A method according to Claim 6 wherein said matrix comprises a resin or agarose beads.
The binding to the mannose-coated agarose beads was estimated as follows,.
Lysates were collected after 48hours, immunoprecipitated using myc agarose beads.
Spherical homogeneous agarose beads were obtained with a largest particle diameter of 200µm.
Thus, the complex is easily purified using glutathione agarose beads as outlined below,.
In step ( e ) of the process, the agarose beads may be separated from the hydrophobic liquid.
The HIC was carried out using a resin consisting of cross-linked agarose beads derivatized with phenyl.
The agarose beads used are typically of 4 % and 6 % densities with a high binding capacity for protein.
Most ion-exchange and affinity packed-bed media are based on agarose beads.
Indeed, agarose beads carrying peanut or jacalin lectins are commercially available ( Vector Laboratories ).
Carriers include red blood cells, bacteriophages, proteins, and synthetic particles such as agarose beads.
After incubation at 4°C overnight, protein-A agarose beads were used to isolate the immune complexes.
Supernatant, TNT mixture after incubation with Ni-NTA magnetic agarose beads.
CNBr activated agarose beads ( without enzyme ) blocked with 1M ethanolamine, were used as untreated control.
Commercially available immobilized to agarose beads ( Pierce ).
Ni-NTA agarose beads + scFv were then washed 4X as previously described.
SAP was depleted from plasma using agarose beads ( BioGel A, BioRad ).
The agarose beads are pelleted by brief centrifugation at 800 rpm for 1 minute.
You'll also be interested in:
Examples of using Beads
Show more
Lump charcoal or heat beads are not recommended
Your beads and buckles and bows
I gave you the beads to save you
Examples of using Agarose
Show more
Min the agarose gel is ready to use
Agar consists of a mixture of agarose and agaropectin
And agarose our leave in life after
