Examples of 'sepharose beads' in a sentence
Meaning of "sepharose beads"
Sepharose beads: Small, porous beads made from cross-linked agarose used for protein purification in biotechnology
How to use "sepharose beads" in a sentence
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sepharose beads
Biotinylated product is bound streptavidin sepharose beads.
Sepharose beads were removed from the supernatants by centrifugation.
The proteins were purified by affinity chromatography using glutathione sepharose beads.
Control samples containing sepharose beads not conjugated to novobiocin are done in parallel.
The AB antigen used in these affinity measurements was attached to sepharose beads.
Sepharose beads are added at a concentration at which essentially all antibodies are captured.
Then the Dynal bead mixture was added to the resuspended sepharose beads.
Sepharose beads can be used when the assay is performed with a washing step.
Pneumoniae French Press lysates were run over a column of fibronectin coated sepharose beads.
Only Sepharose beads containing the appropriated DNA fragments were retained.
Radiolabelled medium was precipitated with Protein-A sepharose beads.
The Sepharose beads were removed by centrifugation and the supernatant applied to a PAGE.
Briefly, soluble tissue factor was covalently coupled to sepharose beads by bromine cyanide.
The protein G sepharose beads were then washed three times with lysis buffer.
Suitable carriers include ovalbumin, keyhole limpet hemocyanin, bovine serum albumin, sepharose beads and polydextran beads.
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Suspend reversed sepharose beads in 1 mL of waterfree DMF.
In a preferred embodiment, the capture beads are Sepharose beads.
The sepharose beads were washed extensively in lysis buffer, then in PBS.
The secreted HA protein was purified in a single step protocol by using Strep-tactin sepharose beads.
Micron NHS Sepharose beads were coupled with 1 mM of each of the following primers,.
The receptor / Fc constructs were precipitated by incubation with Protein A sepharose beads.
Sepharose beads were spun down and washed three times with 1 ml cold PBS.
In the first experiment, the neublastin was extracted with mGFRa3-Ig attached to Sepharose beads.
The Sepharose beads are boiled for 5 minutes to dissociate the immunocomplexes from the beads.
SH53 protein was purified from the supernatant using Strep-tactin sepharose beads.
These sepharose beads containing the bound PCR product were purified, washed, denatured and washed again.
After the incubation, the protein A sepharose beads were removed and added to STF293 cells.
The Sepharose beads are centrifuged, washed and eluted with SDS dissociation buffer.
In one example DNA is bound non-covalently to a diethylaminoethyl - sepharose beads ( DEAE sepharose, GE healthcare ).
SBI covalently cross-linked to Sepharose beads inhibited the MIR and mitogen stimulation virtually completely.
Streptavidin Mag Sepharose ( GE Life Sciences ), streptavidin coupled to magnetite-containing sepharose beads.
MGFRa3-Ig was attached to Sepharose beads using the conditions suggested by the manufacturer, Pharmacia Inc.
Protein was immunoprecipitated from lysates of transfected cells using IM140 coupled to Protein A Sepharose beads.
Preferably, the capture beads are Sepharose beads approximately 25 to 40 µm in diameter.
The method of claim 1 or 14, wherein the beads are sepharose beads.
The Sepharose beads are resuspended in 150 µl 2 x sample buffer and mixed gently.
The method of embodiment 1 or 22, wherein the beads are sepharose beads.
In a preferred embodiment, the capture beads are Sepharose beads approximately 25 to 40 µm in diameter.
The CDX-transfected COS 7 cells also form rosettes with a-CDX-coated sepharose beads.
Presentation column Goat anti-mouse Sepharose beads were packed in a disposable column ( 0.5 ml packed volume ).
Protein A sepharose beads were added, and allowed to incubate at 4°C for 1 h.
Add 50µL of protein A sepharose beads.
After annealing, the sepharose beads were washed three times with Annealing Buffer containing 0.1 % Tween 20.
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Examples of using Sepharose
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In one embodiment the solid support is sepharose
Sepharose has been found to be suitable for this purpose
Enzyme was purified by means of a glutathione sepharose column
Examples of using Beads
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Lump charcoal or heat beads are not recommended
Your beads and buckles and bows
I gave you the beads to save you
