Examples of 'allele specific' in a sentence
Meaning of "allele specific"
allele specific - Refers to a situation where particular alleles (different forms of a gene) are specifically targeted, analyzed, or considered in a genetic context
How to use "allele specific" in a sentence
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allele specific
Allele specific primers can also be used.
Further herein isolated allele specific oligonucleotides are provided.
Allele specific oligoprobe.
In another embodiment of the invention isolated allele specific oligonucleotides are provided.
The allele specific product generation is executed by an oligonucleotide ligation assay.
The first step probe may be either an allele specific probe or locus specific probe.
The allele specific probe may contain a allele specific identifier.
The present disclosure is also directed towards a dual allele specific primer design strategy.
A number of allele specific motifs have been identified.
Targeted genotyping can be performed by allele specific PCR.
Primers for allele specific amplification.
In one case, we were able to demonstrate that the assay was allele specific.
The two allele specific genotyping oligonucleotides may be packaged in separate containers.
Primer extension is done with allele specific primers and a DNA polymerase.
The allele specific product generation is also executed by a flap endonuclease reaction.
See also
Taqman EPR assay development was based upon discovered allele specific SNPs of the sequence panel.
The generation of the allele specific products is performed by using allele specific oligonucleotides.
Detection of the mutation by TagMan ® allele specific amplification.
Two of them contain a allele specific sequence and one is employed as the ligation partner.
Detection of the mutation by TaqMan ® allele specific amplification.
The allele specific probes are only capable of hybridizing without mismatches to the appropriate site.
The use of microarrays comprising allele specific oligonucleotides is described in more detail below.
Allele specific PCR is then used to enrich rare alleles.
In this case the generation of allele specific products is achieved by a Flap endonuclease reaction.
Allele specific PCR amplification products of three individuals.
That is, either locus specific or allele specific primers are hybridized with a target.
The allele specific primer is designed to match one allele of a heterozygous SNP.
In another embodiment, said sequencing comprises allele specific hybridization to a library of labeled oligonucleotide probes.
Allele specific oligonucleotide hybridization ;.
The method that can be used are primer extension, allele specific ligation, or cleavage reaction.
The two allele specific primers carry a tag of unique sequences that determine each allele.
Most preferably, the composition comprises at least two sets of allele specific primer pairs.
Allele specific enrichment with a blocking probe is affected by the annealing / extension temperature.
Other suitable methods include the development of allele specific oligonucleotides ( ASOs ) for specific mutational events.
An allele specific hybridization ( ASH ) probe is an example of a preferred probe.
In addition, a technique known as allele specific PCR can be used.
The allele specific oligonucleotides ( ASO ) were designed with the polymorphism approximately in the middle.
Targeted genotyping can be performed by allele specific oligonucleotide ( ASO ) probes.
Alternatively, allele specific amplification technology which depends on selective PCR amplification may be used.
Data were analyzed to identifydifferentially expressed genes and allele specific expression ( ASE ).
An exemplification of the dual allele specific primer design strategy is given in FIG . 5.
The technique requires that the terminal 3 ' - nucleotide of the PCR primer be allele specific.
To increase the specificity of allele specific PCR, two major approaches are taken.
Allele specific DSA and could not resolve typing to allele 1 1 2.
PCR products are created with a 3 ′ mutant allele specific primer.
FIG . 5 shows the dual allele specific primer design strategy of the present invention.
Allele specific PCR ( 1 . B ) leads to the amplification of the paternal strand.
That is, the specificity component is an allele specific step ( e.g. genotyping or SNP analysis ).
Fig . 9 shows allele specific oligonucleotide detection of the Zmax1 exon 3 mutation.
In some embodiments, the allele specific primer is SEQ ID NO, 5.
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