Examples of 'allele-specific' in a sentence
Meaning of "allele-specific"
allele-specific (adjective) - This phrase is commonly used in genetics to describe a characteristic or process that is specific to one allele or gene variant
How to use "allele-specific" in a sentence
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allele-specific
A polynucleotide probe may be an allele-specific probe.
Allele-specific polymerase chain reaction.
Constructs to assess allele-specific silencing.
Typically, allele-specific oligonucleotides are utilized in performing such methods.
These two enzymes cleave in an allele-specific manner.
Examples of allele-specific probes that can be used in a kit of the invention are,.
These are discussed above and referred to as allele-specific probes.
Design of successful allele-specific primers is an unpredictable art.
The present invention is particularly suitable for allele-specific amplification.
A plurality of allele-specific detection polynucleotides can be used to detect a plurality of different SNPs.
A variation of this approach is the allele-specific primer extension.
Allele-specific oligonucleotide primers hybridizing to either the coding or noncoding strand are contemplated.
In another embodiment, the complement of an allele-specific oligonucleotide is provided.
One of the probes is for instance fluorescently labeled and allele-specific.
Nucleic acid amplification with allele-specific suppression of sequence variants.
See also
The specific amplification requires the presence af PPi and allele-specific template.
Blocking probe competes with the allele-specific primer for increased sensitivity.
An allele-specific motif and algorithms were determined and quantitative binding assays were carried out.
Nucleic acid amplification method using allele-specific reactive primer.
Allele-specific amplification reactions and allele-specific extension reactions are well known in the art.
In another embodiment, a microarray comprises an allele-specific oligonucleotide or its complement.
The allele-specific oligonucleotides will only significantly hybridize if the allele is present in the sample.
In another embodiment, a microarray comprising an allele-specific oligonucleotide or its complement is provided.
Allele-specific probes can be designed for the other disclosed probe type in a similar fashion.
In some embodiments, gene mutations are detected using an allele-specific amplification assay.
Figure 2 shows the results of allele-specific amplification using primers with internal base modifications.
In a preferred embodiment, the set of oligonucleotides in the kit are allele-specific oligonucleotides.
If fluorescence is observed from both allele-specific probes, the sample is heterozygous for both alleles.
Another notable advantage of ligase chain reaction is the capacity for allele-specific amplification.
Under stringent conditions, the allele-specific primer will only be extended on the complementary target.
The few cases which do arise can be resolved using allele-specific amplification.
If desired, a second allele-specific oligonucleotide probe that matches an alternate allele also can be used.
The ApoE genotype was determined using an allele-specific primer extension method.
The allele-specific amplification product is generated in the same reaction vessel without opening the tube.
Probes are labelled at both ends with an allele-specific dye and a quencher.
The allele-specific amplification assay may employ any suitable nucleotide-incorporating biocatalyst known in the art.
Polymorphisms are also commonly detected using allele-specific amplification or primer extension methods.
The mismatched target forms a mismatch with the selective nucleotide of each of the allele-specific primers.
Ideally, primer extension occurs only when the allele-specific primer is hybridized to the target sequence.
Allele-specific analysis of DNA replication origins in mammalian cells.
In another aspect, the present invention is a method of allele-specific amplification of a target nucleic acid.
The allele-specific probe will contain a base complementary to the SNP nucleotide.
The resulting plaques are hybridized with kinased synthetic primer under allele-specific conditions.
It uses allele-specific oligonucleotides to fractionate DNA molecules differing by a single base substitution.
The microarray comprised a modified crystal surface to which allele-specific oligonucleotide probes were covalently attached.
For example, an allele-specific oligonucleotide is applied to immobilized oligonucleotides representing SNP sequences.
In another aspect, the invention provides kits for conducting allele-specific amplification according to the invention.
For an allele-specific PCR assay, any thermostable nucleotide incorporating biocatalyst may be used.
In another embodiment, the invention provides kits for conducting allele-specific amplification according to the invention.
Whereas PCR amplifies both alleles for a particular variant, the ligation process of LCR is allele-specific.
