Examples of 'antisense primer' in a sentence
Meaning of "antisense primer"
antisense primer: In molecular biology, an antisense primer is a short piece of single-stranded DNA or RNA that is complementary to a target mRNA sequence. It is used in techniques like PCR to synthesize the DNA strand that is complementary to the target mRNA, allowing for further analysis or manipulation of gene expression
How to use "antisense primer" in a sentence
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antisense primer
The above are requirements for an antisense primer.
Inner antisense primer.
DNA sequences in bold show the sense primer and in italics the antisense primer.
Antisense Primer to mutagenize the Aspergillus nigerAMG terminator sequence,.
These oligonucleotides are particularly useful as an antisense primer for amplifying a HBV nucleic acid.
The antisense primer contains a TAA stop codon and a Bam HI site ( underlined ).
The área sandwiched between the sense primer and the antisense primer is amplified by PCR.
The antisense primer B primes at the 3 ' end of the S gene.
A SpeI site was engineered at the 5 ′ end of the antisense primer to facilitate ligation.
Antisense primer for both variants, named 2B2, corresponding to a common region located at exon 9,.
SEQ ID NO, 4 is the nucleotide sequence for an antisense primer for adhE deletion.
An antisense primer ( hereinafter referred to as " primer ( F ) ) complementary to the primer ( E ) is synthesized.
SEQ ID NO, 2 is the nucleotide sequence for an antisense primer for frdBC deletion.
The HPV6E7 antisense primer shares some homology with HPV2.
SEQ ID NO, 6 is the nucleotide sequence for an antisense primer for celY deletion.
See also
A PCR is conducted using the aforesaid template DNA, sense primer, and antisense primer.
All primer mixes contain the antisense primer at the 3 ' - end named DRBAS.
A SpeI site was engineered into the 5 ′ end of the antisense primer.
At that time, the antisense primer ( GSP1 ) shown below was used.
A Spe I site was engineered into the 5 ' end of the antisense primer.
A Spe I site was engineered at the 5 ' end of the antisense primer to facilitate ligation.
SEQ ID NO, 8 is the nucleotide sequence of a degenerate antisense primer.
Similarly, T3 primer and each antisense primer were used for PCR.
Primers used herein are as follows . " S " indicates a sense primer, and " AS " indicates an antisense primer.
SEQ ID NO, 10 is the nucleotide sequence for an antisense primer for amplification of FRT-cat-sacB.
The vector primer SEQ ID NO, 16 served as the antisense primer.
SEQ ID NO, 20 is the nucleotide sequence for an antisense primer for cloning mgsA.
SEQ ID NO, 14 is the nucleotide sequence for an antisense primer for cloning frdABCD.
SEQ ID . NO, 8 is the nucleotide sequence for an antisense primer for mgsA deletion.
SEQ ID NO, 16 is the nucleotide sequence for an antisense primer for cloning ackA.
SEQ ID NO, 28 is the nucleotide sequence for an antisense primer for deleting ackA.
SEQ ID NO, 36 is the nucleotide sequence for an antisense primer for deleting adhE.
SEQ ID NO, 24 is the nucleotide sequence for an antisense primer for cloning adhE.
SEQ ID NO, 32 is the nucleotide sequence for an antisense primer for deleting mgsA.
SEQ ID NO, 26 is the nucleotide sequence for an antisense primer for deleting frdBC.
SEQ ID NO, 34 is the nucleotide sequence for an antisense primer for deleting focA-pflB.
SEQ ID NO, 22 is the nucleotide sequence for an antisense primer for cloning focA-pflB.
SEQ ID NO, 18 is the nucleotide sequence for an antisense primer for cloning lacZ-cynX.
SEQ ID NO, 29 is the nucleotide sequence for a lacZ antisense primer with 5 ' NheI site.
SEQ ID NO, 30 is the nucleotide sequence for a cynX antisense primer with 5 ' NheI site.
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Examples of using Antisense
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Antisense RNAs can be classified in different ways
In a separate embodiment new antisense oligodeoxynucleotides are provided
Antisense polynucleotides can include deoxyribonucleotides or ribonucleotides
Examples of using Primer
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The primer includes the following modules
It was mostly gray primer and fender fill
A primer is not necessary for small repairs