Examples of 'blunt ended' in a sentence
Meaning of "blunt ended"
blunt ended: This phrase describes something that has a flat or dull tip or edge, rather than being sharp or pointed
How to use "blunt ended" in a sentence
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blunt ended
Blunt ended molecules may also be used.
Restriction fragments can be blunt ended or have an overhang.
The blunt ended vector is dephosphorylated.
Both EcoRV and HincII are blunt ended sites.
The ligation may be blunt ended or vía use of complementary over hanging ends.
The other end of the siRNA dicer substrate is blunt ended.
Adaptor molecules are preferably defined as blunt ended double stranded oligonucleotides.
The blunt ended hY-IFN is isolated by agarose gel electrophoresis.
The HpaI site can be used to clone blunt ended fragments.
This digest produces blunt ended fragments with an average length of 64 nucleotides.
The gel is applied into the periodontal pocket through a blunt ended cannula.
Single stranded siRNA as well as blunt ended forms of dsRNA can also be used.
Further, the template polynucleotide molecules can be blunt ended.
The remaining backbone was blunt ended by Klenow and religated.
The instrument may be a pointer, for example a blunt ended rod.
See also
The fragment was made blunt ended using Klenow fragment A.
The dsRNA of the invention may be blunt ended e . g.
Blunt ended up using a northern accent, and it did the trick, her stammer disappeared.
The RNA molecule may be double stranded and either blunt ended or comprises overhanging ends.
Additionally, it is to be noted that these particular siRNA compounds are also blunt ended.
It is digested with HindIII, blunt ended and then digested with EcoRI.
The piercing elements include, for example, pointed needle cannula or blunt ended cannula.
These two blunt ended fragments were ligated together to produce the clone pNBOXOX1.
In another embodiment, the siRNA complex is blunt ended at both ends.
Hairpin adaptors find use in blunt ended ligation to yield self-priming template strands.
Each of these unique restriction sites results in an in-frame blunt ended digestion product.
Alternatively, blunt ended adaptors can be used, in the case of blunt ended restriction fragments.
In one embodiment, the material is penetrable by blunt ended needles or pipetting devices.
The isolated blunt ended hY-IFN DNA is phosphorylated using polynucleotide kinase.
The piercing elements include, for example, pointed needle cannulae or blunt ended cannulae.
The cDNA was blunt ended by the action of T4 DNA polymerase.
In another embodiment, the RNA duplex is blunt ended in both ends.
A blunt ended pipetting device 32 is also shown.
In another embodiment, the RNA complex is blunt ended at both ends.
Then, the fragment was blunt-ended with T4 DNA polymerase to afford a first blunt ended DNA fragment.
Prior to ligation, the fragment is optionally blunt ended with DNA polymerase.
This yields four blunt ended fragments per molecule of pBR322.
The KMX2 fragment was isolated from an agarose gel and blunt ended with Klenow enzyme.
The sheared DNA is made blunt ended and then ‘ A ' overhangs are generated.
PBluescript with full length MN cDNA was cleaved by EcoRI and blunt ended by S1 nuclease.
The inserts were made blunt ended and cloned into pJR1 and pMK4.
The method of claim 7, wherein the pipetting device is a blunt ended needle.
A hammer and screwdriver, or other blunt ended tool ( not included ) are needed for assembly.
The corresponding adaptors ( see below ) then may also be blunt ended.
Fragments are made are made blunt ended using S1 nuclease.
Blunt ended molecules were then ligated and electrotransformed into MC1061.
EcoRI adapters were ligated to the blunt ended cDNA by T4 DNA ligase.
The blunt ended PSE enhancer / promoter was ligated to SpeI linearized, blunt ended vector CN188.
Plasmid pTRKH4 was digested with Xba I and subsequently blunt ended with the Klenow fragment.
After his relationship with Blunt ended in July 2008, Bublé began dating actress Luisana Lopilato.
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Examples of using Blunt
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Blunt trauma to the chest and abdomen
No obvious signs of blunt or sharp force trauma
Blunt force trauma to the temporal bone
Examples of using Ended
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You ended up doing something you should not
We could have all ended up in the can
All we ended up doing is creating a new breed of roaches
